抗精神分裂症药物对吐鲁番斗鸡5-HTT和TPH1基因表达的影响

为探讨不同抗精神分裂症药物对吐鲁番斗鸡攻击行为影响的分子机理，试验将36只斗鸡随机分为12组，并选取利培酮、阿立哌唑、盐酸曲唑酮、盐酸舍曲林4种抗精神分裂症药物，且每种药物设有低剂量组（L）、正常剂量组（N）及高剂量组（H），每天人工口服给药。分别在第1、2、5、8、11、14天时，翅下静脉采血，并采用实时荧光定量PCR法对口服不同剂量药物的吐鲁番斗鸡各阶段血液中5-羟色胺转运体（5-hydroxy-tryptamine transporter，5-HTT）、色氨酸羟化酶1（tryptophan hydroxylase 1，TPH1）的mRNA表达量进行检测。结果显示，口服4种药物后，利培酮组和阿立哌唑组斗鸡血液中5-HTT、TPH1基因mRNA的表达变化具有相似性，5-HTT基因整体呈下降趋势，TPH1基因均表现为波动性上升，且利培酮组TPH1基因mRNA的表达量及上升幅度高于阿立哌唑组；盐酸曲唑酮组和盐酸舍曲林组5-HTT基因mRNA的表达均从第5天开始呈直线上升，并于第14天达到峰值，且盐酸舍曲林组TPH1基因的表达呈微上调，盐酸曲唑酮组则无明显变化。利培酮组和阿立哌唑组斗鸡攻击行为减弱与其血液中5-HTT基因mRNA的低表达有关，本试验结果为进一步研究药物影响吐鲁番斗鸡攻击行为的分子机理提供了重要参考依据。

Effects of Antipsychotic Schizophrenia Drugs on the Expression of 5-HTT and TPH1 Genes in Turpan Cockfighting

In order to investigate the molecular mechanism of different antischizophrenic drugs on the aggressive behavior of Turpan cockfighting. A total of 36 cockfightings were randomly divided into 12 groups,and four antipsychotic drugs of risperidone,aripiprazole,trazodone hydrochloride,sertraline hydrochloride were selected,and each drug was given a low dose group (L),normal dose group (N) and high dose group (H) with daily artificial oral medication. Respectively,at the 1,2,5,8,11 and 14 days,the blood samples were collected from wing vein,and the mRNA expression of 5-HTT and TPH1 genes in the blood of Turpan cockfighting at various stages of oral administration in different drugs doses were measured by quantitative Real-time PCR. The results showed that the expression change of 5-HTT and TPH1 genes mRNA in the blood of the risperidone group and aripiprazole group were similar. The overall 5-HTT gene showed a downward trend,and the TPH1 gene showed increased volatility,and the expression of TPH1 gene mRNA in risperidone group was higher than that in aripiprazole group.The expression of 5-HTT gene mRNA in the trazodone hydrochloride group and sertraline hydrochloride group increased linearly from the 5th day and peaked at the 14th day, and the TPH1 gene in the sertraline hydrochloride group was slightly up-regulated,but there was no obvious change in the trazodone hydrochloride group. It was suggested that the molecular influencing factors of the decrease in the aggressive behavior of risperidone and aripiprazole groups were related to the low expression of 5-HTT gene in the blood,and the results of this study could provide an important reference for further study on the molecular mechanism of the drug affecting the aggressive behavior of Turpan cockfighting.