复合微生态制剂、饲用抗生素对肉鸡生长性能、肠道菌群数量和免疫性能的影响

本试验旨在研究复合微生态制剂及饲用抗生素对肉鸡生长性能、肠道菌群数量和免疫性能的影响。选择体重接近、健康的600只1日龄白羽肉雏鸡随机分成5个组，每组6个重复，每个重复20只。Ⅰ组为对照组，饲喂基础日粮；Ⅱ组为肽菌素T3组，在基础日粮中添加1.0‰的复合微生态制剂肽菌素T3；Ⅲ、Ⅳ、Ⅴ组为抗生素组，分别在基础日粮中添加20 g/t硫酸黏杆菌素、50 g/t杆菌肽锌及50 g/t杆菌肽锌和10 g/t硫酸黏杆菌素混合物。预试期3 d，正试期35 d。结果表明，与Ⅰ、Ⅲ、Ⅳ和Ⅴ组相比，肽菌素T3组肉鸡各阶段平均日增重（ADG）、盲肠和直肠乳酸杆菌数量显著增高（P<0.05），料重比（F/G）、盲肠和直肠大肠杆菌数量显著降低（P<0.05）；脾脏指数分别提高27.44%、40.27%、29.01%和29.81%（P<0.05），法氏囊指数分别提高12.82%、37.50%、33.33%和38.95%（P<0.05）。肽菌素T3组肉鸡血清内毒素含量低于Ⅰ组（P>0.05）和Ⅲ组（降低24.77%，P<0.05）。与Ⅰ组和Ⅲ组相比，肽菌素T3组盲肠内容物分泌型免疫球蛋白A（sIgA）含量分别提高32.37%和25.79%（P<0.05）。综合上述结果，本试验条件下，日粮中添加1.0‰肽菌素T3可提高肉鸡生长性能，改善肉鸡肠道菌群结构，促进肉鸡脾脏和法氏囊的发育，其提高盲肠内容物sIgA含量、降低血清内毒素含量的效果优于20 g/t硫酸黏杆菌素。

Effects of Compound Probiotics and Antibiotics on Growth Performance,Intestinal Flora and Immune Function of Broilers

This trial was conducted to evaluate the effects of compound probiotics and antibiotics on growth performance,intestinal microflora and immune function of broilers.Six hundred one-day-old female broiler chickens were randomly divided into 5 groups with 6 replicates per group and 20 broilers per replicate.The broilers in groupⅠ were fed basal diet,and the basal diet+1.0‰ T3(compound probiotics),the basal diet+20 g/t colistin sulphate,the basal diet+50 g/t bacitracin zinc,the basal diet+50 g/t bacitracin zinc+10 g/t colistin sulphate were provided to the broilers in group Ⅱ,Ⅲ,Ⅳ and Ⅴ,respectively.The pre-trial period lasted for 3 days,and the experiment lasted for 35 days.The results showed as follows:Compared with group Ⅰ,Ⅲ,Ⅳ and Ⅴ,the average daily gain (ADG) at each trial stage,the number of Lactobacillus in cecal and rectal were significantly increased (P<0.05),while the feed gain ratio (F/G),the number of Escherichia coli in cecal and rectal were significantly decreased in T3 group.Compared with group Ⅰ,Ⅲ,Ⅳ and Ⅴ,the spleen index of the group treated with T3 increased 27.44%,40.27%,29.01% and 29.81% (P<0.05);The bursa of Fabricius index increased significantly by 12.82%,37.50%,33.33% and 38.95%,respectively (P<0.05);The content of serum endotoxin in T3 group was lower than that of group Ⅰ(P>0.05) and group Ⅲ (24.77%, P<0.05).Compared with the groupⅠ and Ⅲ,the content of secreted immunoglobulin A (sIgA) in T3 group was significantly increased by 32.37% and 25.79%(P<0.05).In conclusion,adding 1.0‰ T3 could improve the growth performance and intestinal flora,promote the development of spleen and bursa in broilers.And the role of increasing the content of sIgA in cecal contents,decreasing the content of serum endotoxin of 1.0‰ T3 was better than the use of 20 g/t of clostridium sulfate.