1例鸡白痢沙门氏菌的分离鉴定与耐药性分析

为查明河南省新乡市某规模化蛋鸡场疑似沙门氏菌感染病例的病原，进而制定合理的治疗方案，本研究无菌采集疑似病雏鸡肠管样品，用革兰氏染色、培养特性观察、生化试验、PCR方法对其进行确诊，并进行病原回归试验，采用药敏纸片琼脂扩散法（K-B法）分析分离菌对19种常见抗生素的耐药性。结果显示，本试验成功分离了一株革兰氏阴性短杆菌，该分离菌符合鸡白痢沙门氏菌的培养特性和生化特性；PCR成功扩出invA基因，通过与GenBank数据库比对分析确定该细菌为鸡白痢沙门氏菌；用分离细菌株感染SPF鸡能复制出与自然感染一致的病例，说明鸡白痢沙门氏菌是造成本养鸡场雏鸡发病的主要病原；分离株具有较强的致病性和多重耐药性，对阿米卡星、苯唑青霉素、青霉素耐药，对复达欣、氨苄青霉素、头孢曲松等药物敏感，将敏感抗生素用于临床治疗效果明显。结合以上结果，本研究提出该病的具体防治措施，并取得了较好的效果，为鸡白痢沙门氏菌的分离鉴定及防治提供了参考，对鸡白痢沙门氏菌病病原的早期诊断和治疗具有重要临床意义。

Isolation,Identification and Analysis of Drug Resistance of Salmonella Pullorum

The study was aimed to investigate the pathogens of a suspected Salmonella infection from a large-scale layer farm in Xinxiang city Henan province,and provide a reasonable treatment.The intestinal tube samples of sick chickens were collected under sterile condition,and gram staining,culture characteristics,biochemical test and PCR were carried out,the pathogen regression was performed,also,the drug sensitive of 19 kinds of common antibiotics were analyzed using the disk diffusion (K-B) method.The results showed that a strain of gram-negative bacillus was successfully isolated,and consistent with the culture characteristics and pathologic characteristics of Salmonella Pullorum,the invA gene was amplified successfully by PCR,and the strain was identified as Salmonella Pullorum by comparison with the GenBank database;Using isolated bacterial strain to infect SPF chickens could replicate cases consistent with natural infections.It indicated that the Salmonella Pullorum was the main pathogenic agent of the chickens in this chicken farm.The pathogenic and multiple drug resistance of the Salmonella Pullorum were strong.The isolate strain was resistant to amikacin,oxacillin and penicillin,but it was sensitive to fortum (ceftazidime),ampicillin and ceftriaxone,which could be recommended for clinical treatment positive effect.Above all,this study proposed the specific prevention and treatment measures for the disease and achieved good results.This study initially provided a reference for the diagnosis and treatment of Salmonella Pullorum,with great clinical significance in the early diagnosis and treatment of pathogens of Salmonella Pullorum.