Effect of Aurora protein kinase inhibitor VX-680 on homogeneity adhesion and migration in human hepatoma HepG2 cell

AIM:To study the effect of Aurora protein kinase inhibitor VX-680 on homogeneous adhesion and migration ability in human hepatocellular carcinoma cell line HepG2. METHODS:The HepG2 cell were divided into experimental group and control group, respectively. VX-680 was used in experimental groups at 3 concentrations (3.125 μmol/L group, 6.25 μmol/L group and 12.5 μmol/L group). DMSO was used in the control group. The effects of VX-680 at different concentrations on the adhesion ability of human hepatocellular carcinoma HepG2 cells were observed by cell slow aggregation test and separation experiment. The effects of VX-680 at different concentrations on the migration ability of HepG2 cells was detected by wound healing assay. The expression of E-cadherin in HepG2 cells was detected by Western blot. RESULTS:The results of the slow aggregation test showed that compared with the control group, the number of cell clumps formed in experimental groups was significantly decreased (P<0.01). The results of separation experiment showed that the ratio of N_TC/N_TE gradually decreased with the increased concentration of VX-680. The results of wound healing assay showed that as the concentration of VX-680 increased, the cell scratch healing ability gradually weakened compared with control group. The results of Western blot showed that the protein expression of E-cadherin in the HepG2 cells increased with the increased concentration of VX-680 (P<0.05). CONCLUSION:VX-680 increases the homogeneous adhesion and inhibits the migration of HepG2 cells.