Adipophilin promotes intracellular lipid accumulation through PI3K/Akt signaling pathway |
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Authors: | LIU Qing-nan DAI Zhi-bing YUAN Zhong-hua LIU Yuan-yue TAN Gui-huang XU Gui-na HE Xu |
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Affiliation: | 1. Yiyang Medical College, Yiyang Central Hospital, Yiyang 413000, China;2. Department of Ultrasonography, Yiyang Central Hospital, Yiyang 413000, China;3. Institute of Cardiovascular Disease, Key Laboratory for Arteriosclerology of Hunan Province, University of South China, Hengyang 421001, China |
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Abstract: | AIM:To observe the possible mechanism through which adipophilin promotes the accumulation of intracellular lipids, and to provide a reference for controlling atherosclerosis.METHODS:RAW264.7 cells were incubated with oxidized low-density lipoprotein (oxLDL) for different time. qPCR, Western blot and Oil red O staining were used to observe the mRNA and protein levels of Akt, p-Akt and adipophilin and lipid accumulation. The above indexes were measured after the cells were treated with PI3K/Akt signaling pathway inhibitor LY294002. The activation of Akt was analyzed in the HEK293 cells over-expressing adipophilin. Co-immunoprecipitation was applied for analysis of protein-protein interaction between adipophilin and Akt. RESULTS:After incubation with oxLDL, the amount of lipid droplets, Akt activity and adipophilin expression increased in the cells with the extension of time (P<0.05). Moreover, LY294002 inhibited the above changes. The p-Akt levels increased after adipophilin over-expression. No direct interaction between adipophilin and Akt proteins was observed. CONCLUSION:Adipophilin promotes the accumulation of intracellular lipids through PI3K/Akt signaling pathway, but possibly not by direct interaction between adipophilin and Akt proteins. |
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Keywords: | Adipophilin Lipid accumulation PI3K/Akt signaling pathway Atherosclerosis |
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