Exogenous hydrogen sulfide attenuates obstructive kidney injury in mice |
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Authors: | WANG Hong-gang CHENG Hui-jun WU Dong-dong WANG Jun LIU Zheng-guo JI Ai-ling LI Yan-zhang |
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Institution: | Basic Medical College of Henan University, Kaifeng 475004, China |
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Abstract: | AIM:To investigate the protective effect of exogenous hydrogen sulfide (H2S) on obstructive renal injury in mice, and to explore the possible potential mechanisms involved in this animal model. METHODS:Male C57BL/6 mice (8 weeks old) were randomly divided into sham group, operation group and H2S group, with 5 rats in each group. The model of obstructive renal injury was induced by unilateral ureteral obstruction (UUO). The mice in H2S group were intraperitoneally injected with NaHS daily, while the mice in sham group and operation group were administered with the same volume of saline intraperitoneally. After 7 d, the mice were executed and the renal tissues were taken out for experiments. RNA was extracted to detect the mRNA expression of H2S catalytic enzymes in the mice of 3 groups. HE staining was performed to observe the structural changes of renal tissues in the mice. Renal fibrosis in the mice of 3 groups was evaluated by Masson staining. The content of cystatin C in the plasma was detected to reflect glomerular filtration ability. The protein expression of LC3, beclin-1 and fibronectin (FN) in the mice of 3 groups was determined by Western blot. RESULTS:Compared with sham group, the mRNA expression of cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE) and 3-mercaptopyruvate sulfurtransferase (3-MST) in operation group decreased significantly. The collagen fiber content in operation group was increased significantly, while collagen fiber content in H2S group was decreased significantly as compared with operation group. Compared with sham group, the protein expression of FN in operation group was increased significantly, while the protein expression of FN in H2S group was decreased significantly as compared with operation group. Compared with sham group, the protein expression of LC-Ⅱ and beclin-1 in operation group was increased significantly, while the protein expression of LC-Ⅱ and beclin-1 in H2S group was increased significantly as compared with the operation group. CONCLUSION:Exogenous H2S possibly mitigates renal fibrosis in UUO mice by up-regulating autophagy. |
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Keywords: | Hydrogen sulfide Obstructive renal injury Fibrosis Autophagy |
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