2006－2016年中国羊口疮病毒的遗传演化分析

羊口疮（Orf disease）是由羊口疮病毒（Orf virus，ORFV）引起的人兽共患传染病，该病广泛分布于世界各地，对公共卫生造成了严重的影响。为了解中国ORFV毒株的起源、分子流行病学及病毒系统研究的信息，本研究对中国2006-2016年分离到82株ORFV毒株进行了系统的遗传演化分析，利用DNAStar和Mega 5.0软件对ORFV011（B2L）、ORFV020（VIR）、ORFV059（FIL）和ORFV127（vIL-10）基因序列进行核苷酸和氨基酸同源性比对，并在此基础上构建系统发育树。结果显示，82株病毒B2L基因核苷酸序列和氨基酸序列同源性分别为95.9%~100%和86.5%~100%，F1L基因核苷酸序列和氨基酸序列同源性分别为94.5%~100%和94.0%~100%；系统进化分析显示，中国不同地方分离的ORFV毒株处于不同分支上，甚至同一个省内的毒株也分布在不同分支上。表明中国ORFV毒株进化特殊，处于不同的进化分支上，且有发生突变的可能性，世界各地流行的代表性毒株在中国均有变种分布，这增加了中国防控ORFV的难度。本研究结果为中国防控羊口疮的流行和研制高效疫苗提供了基础数据。

Genetic and Phylogenetic Analysis of Orf Virus Isolated in China from 2006 to 2016

Orf disease, an important zoonotic infectious disease, is caused by the Orf virus (ORFV), which was worldwide distribution and causing serious public health impacts. In order to know the origin, molecular epidemiology and systematic research information of ORFV of China, 82 Chinese ORFV strains were retrieved and performed the genetic phylogenetic analysis,and the homologous alignment of nucleotide and amino acid sequences of ORFV011(B2L), ORFV020(VIR), ORFV059 (F1L) and ORFV127 (vIL-10) were analyzed using DNAStar and Mega 5.0 softwares, the phylogenetic tree was constructed. The results showed that the ORFV011 (B2L) and ORFV059 (F1L) sequences of 82 ORFV strains shared 95.9% to 100% and 94.5% to 100% nucleotide identities and 86.5% to 100% and 94.0% to 100% deduced amino acid identities, respectively. The phylogenetic analysis revealed that Chinese ORFV strains with different region clustered in different branch, even in the same provinces. These results indicated that ORFV strains in China did not form a unique cluster having possibility of mutation occurrence at any time and other worldwide ORFV strains showed variation circulating in China. This was the important obstacles for prevention and control of ORFV. These results provided the basic data for prevention of Orf disease and development of effective vaccine.