Antagonistic effect of thalidomide on TGF-β1-induced change of CTGF gene promoter-binding proteins in HELF cells |
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Authors: | LI Xiao-xiao WANG Da-an LU Da-xiang WANG Hua-dong WEI Wei WANG Yan-ping |
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Institution: | Department of Pathophysiology, Key Laboratory of State Administration of Traditional Chinese Medicine, College of Basic Medicine, Jinan University, Guangzhou 510632, China |
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Abstract: | AIM:To investigate the antagonistic effect of thalidomide (THD) on the activation of connective tissue growth factor (CTGF) gene promoter induced by transforming growth factor-β1 (TGF-β1) in human embryonic lung fibroblasts (HELF). METHODS:Luciferase reporter vector driven by CTGF gene promoter was used to detect the effects of TGF-β1 and THD on the activity of CTGF gene promoter, and DNA pull-down assay with CTGF gene promoter as a probe was used to analyze the changes of CTGF promoter-binding proteins under different conditions. RESULTS:TGF-β1 increased the activity of reporter driven by CTGF gene promoter (P<0.01), while THD significantly inhibited TGF-β1-induced increase in the reporter activity dose-dependently (P<0.01). At the same time, THD had inhibitory effect on the TGF-β1-induced change of CTGF gene promoter-binding proteins (P<0.05). CONCLUSION:Regulation of CTGF gene promoter-binding proteins takes part in TGF-β1-induced activation of CTGF gene promoter, while THD has antagonistic effect on this process. |
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Keywords: | Thalidomide Human embryonic lung fibroblasts Connective tissue growth factor DNA-binding protein Transforming growth factor-β1 |
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