屎肠球菌对AA肉鸡血清和肝脏抗氧化功能的影响

试验旨在研究日粮中添加屎肠球菌对爱拔益加（Arbor Acres，AA）肉鸡血清和肝脏抗氧化功能的影响。选取1日龄AA肉鸡600只（公鸡），随机分为5组，每组6个重复。对照组（CON组）饲喂基础日粮；抗生素组（Ant组）在基础日粮中添加0.1%金霉素；低（LEF组）、中（MEF组）、高（HEF组）剂量屎肠球菌组分别在基础日粮中添加50、100、200 mg/kg屎肠球菌。结果表明：①21日龄时，HEF组肉鸡血清中总抗氧化能力（T-AOC）、谷胱甘肽过氧化物酶（GSH-Px）活性均显著高于CON组（P<0.05），Ant组T-AOC显著高于CON组（P<0.05）；42日龄时，MEF组和HEF组血清中丙二醛（MDA）含量显著低于CON组（P<0.05），LEF组血清中超氧化物歧化酶（SOD）活性显著高于CON组和Ant组（P<0.05）。②21日龄时，MEF组和HEF组肉鸡肝脏中T-AOC、GSH-Px活性均显著高于CON组（P<0.05），且HEF组肉鸡肝脏中T-AOC显著高于Ant组（P<0.05）；42日龄时，HEF组肉鸡肝脏中过氧化氢酶（CAT）活性显著高于CON组（P<0.05）。结果提示，屎肠球菌能显著提高21与42日龄血清和肝脏中T-AOC和GSH-Px活性，降低血清中MDA含量，增加抗氧化酶活性，改善肉鸡抗氧化应激能力，且200 mg/kg屎肠球菌添加量在提高血清和肝脏抗氧化能力上与抗生素效果相当。

Effects of Enterococcus faecium on Antioxidant Function in Serum and Liver of Arbor Acres Broilers

The experiment was aimed to investigate the effects of dietary Enterococcus faecium on antioxidant capacity in serum and liver of Arbor Acres (AA) broilers.A total of 600 AA broilers (roosters) with 1-day-old were randomly allocated into 5 groups with 6 replicates per group.Treatments in control group (CON group) were fed a basal diet,antibiotic group (Ant group) was fed a basal diet supplemented with 0.1% chlorotetracycline,the three groups (LEF,MEF and HEF groups) were fed the basal diet supplemented with 50,100 and 200 mg/kg Enterococcus faecium,respectively.The results showed that:① At 21 days of age,the activity of total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-Px) activity in serum of HEF group were significantly higher than that of CON group (P<0.05),T-AOC in the serum of Ant group was significantly higher than that of CON group (P<0.05).At 42 days of age,the contents of malondialdehyde (MDA) in serum of MEF and HEF groups were significantly lower than that of CON group (P<0.05);The activity of superoxide dismutase (SOD) in serum of LEF group was significantly higher than that of CON and Ant groups (P<0.05).② At 21 days of age,the activity of GSH-Px and T-AOC in MEF and HEF groups were significantly higher than that of CON group (P<0.05),and T-AOC in liver of HEF group was significantly higher than that of Ant group (P<0.05).At 42 days of age,the activity of CAT in liver of HEF group was significantly higher than that of CON group (P<0.05).The results showed that the diet added Enterococcus faecium could significantly increase the activity of GSH-Px and T-AOC and decrease the content of MDA in serum and liver,increase the activity of antioxidant enzyme,improve anti-oxidative stress ability of broiler,and the basal diet supplemented with 200 mg/kg Enterococcus faecium was comparable to that of antibiotics in improving the antioxidant capacity of serum and liver.