海藻硫酸多糖和中药复方对鸡胚接种H9N2亚型禽流感病毒的作用

为探讨海藻硫酸多糖（sulfated polysaccharide from algae，SPA）及中药复方提取液（compound extracts of Chinese medicine，CECM）对H9N2亚型禽流感病毒（avian influenza virus，AIV）的作用，将SPA及CECM以3种不同加药方式作用于接种了H9N2亚型AIV的10日龄SPF鸡胚中，无菌收集鸡胚尿囊液，测定血凝效价，检测2种药液对H9N2亚型AIV的预防、治疗及直接灭活作用。结果显示，SPA及CECM的最大安全浓度分别为50和200 mg/mL；3种不同加药方式下，不同浓度SPA及CECM均能极显著降低鸡胚尿囊液中H9N2亚型AIV的血凝效价（P<0.01），以直接灭活作用组的血凝效价降低幅度最大，预防作用组和治疗作用组次之。预防作用组，CECM高剂量组（200 mg/mL）的抗病毒效果显著优于SPA各剂量组（12.5、25和50 mg/mL）及CECM低、中剂量组（50和100 mg/mL）（P<0.05）；治疗作用组，SPA高剂量组（50 mg/mL）及CECM中、高剂量组（100和200 mg/mL）抗病毒效果显著优于SPA低剂量组（12.5 mg/mL）（P<0.05）；直接灭活作用组，SPA及CECM的抗病毒效果均佳，CECM各剂量组的抗病毒效果与SPA各剂量组差异极显著（P<0.01）。结果表明，SPA和CECM对10日龄鸡胚几乎无毒副作用；SPA及CECM均能显著抑制H9N2亚型AIV在鸡胚尿囊液中的增殖，以直接灭活作用组的抗病毒效果最佳，其抗H9N2亚型AIV作用的强弱与药物浓度、用药和病毒的感染时间顺序密切相关。

Effect of Sulfated Polysaccharide from Algae and Traditional Chinese Medicine Compound on Inoculation of H9N2 Subtype Avian Influenza Virus in Chicken Embryo

To explore the effects of sulfated polysaccharide from algae (SPA) and the compound extracts of Chinese medicine (CECM) on H9N2 subtype avian influenza virus (AIV),SPA and CECM were applied to the 10-day-old SPF chicken embryos inoculated with H9N2 subtype AIV in 3 different ways.The chicken embryo allantoic fluid was collected aseptically,the blood coagulation titer was measured,and the prevention,treatment and direct inactivation of the two kinds of liquids to H9N2 subtype AIV were tested.The results showed that the maximum safe concentrations of SPA and CECM were 50 and 200 mg/mL,respectively.Under different dosing methods,different concentrations of SPA and CECM could extremely significantly reduce the blood coagulation of H9N2 subtype AIV in chicken embryo allantoic fluid (P<0.01).The hemocoagulation titer of the direct inactivation group decreased the most,the preventive group and the therapeutic group followed.In the preventive group,the anti-viral effect of CECM high dose group (200 mg/mL) was significantly better than that of SPA dose groups (12.5,25,50 mg/mL) and CECM low and medium dose groups (50,100 mg/mL) (P<0.05);SPA high-dose group (50 mg/mL) and CECM medium-and high-dose groups (100,200 mg/mL) were significantly better than SPA low group (12.5 mg/mL) (P<0.05);The direct inactivation group,SPA and CECM both had good antiviral effects,and the antiviral effects of CECM dose groups were extremely significantly different from those of SPA dose groups (P<0.01).The results showed that SPA and CECM had almost no toxic side effects on 10 days old chicken embryos;SPA and CECM could significantly inhibit the proliferation of H9N2 subtype AIV in chicken embryo allantoic fluid,and the antiviral effect of direct inactivation group was the best.The strength of its anti-H9N2 subtype AIV was closely related to the drug concentration,medication and viral infection time sequence.