| 转GFP基因蚕和neor蚕的继代分析 |
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| 引用本文： | 魏玲,周泽扬,郭秀洋,鲁成,向仲怀.转GFP基因蚕和neor蚕的继代分析[J].西南农业大学学报,2002,24(3):281-282,285. |
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| 作者姓名： | 魏玲 周泽扬 郭秀洋 鲁成 向仲怀 |
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| 作者单位： | 西南农业大学农业部蚕桑学重点实验室 重庆400716
(魏玲，周泽扬，郭秀洋，鲁成)，西南农业大学农业部蚕桑学重点实验室 重庆400716(向仲怀) |
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| 基金项目： | 重庆市科委院士基金 (渝科委计 [1 998] 1 9号 )，中华农业科教人才基金 (99- 0 3 -A -I)资助 |
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| 摘 要： | 利用PCR技术对该室采用精子介导法构建的转绿色荧光蛋白基因（GFP）蚕及基因枪法构建的新霉素抗性基因（neo^r)要进行了继代分析检测，结果在转GFP蚕的G2和G3代，转neo^r蚕的G1及G2代，均检测到了阳性个体。这说明所构建的转基因蚕具有相对稳定的遗传性。
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| 关 键 词： | GFP 蚕 neo^r蚕 继代分析 转基因 PCR 精子介导法 转绿色荧光蛋白基因 阳性个体 遗传性 |
| 文章编号： | 1000-2642(2002)03-0281-02 |
PCR SCREENING ANALYSIS OF TRANSGENIC SILKWORM OF GFP AND NEOr |
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| WEI Ling,ZHOU Ze-yang ,GUO Xiu-yang,LU Cheng,XIANG Zhong-huai.PCR SCREENING ANALYSIS OF TRANSGENIC SILKWORM OF GFP AND NEOr[J].Journal of Southwest Agricultural University,2002,24(3):281-282,285. |
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| Authors: | WEI Ling ZHOU Ze-yang GUO Xiu-yang LU Cheng XIANG Zhong-huai |
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| Institution: | WEI Ling,ZHOU Ze-yang *,GUO Xiu-yang,LU Cheng,XIANG Zhong-huai |
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| Abstract: | Positive individuals in both G2 and G3 generations of the transgenic silkworm of GFP (green fluorescent protein) by the sperm-mediated method and in G1 and G2 generations of the transgenic silkworm of neo r (neomycin resistance gene) by the gene gun method were obtained by PCR screening, indicating that the transgenic silkworm by either the sperm-mediated method or the gene gun method has relatively stable transmissibility. |
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| Keywords: | transgenic silkworm PCR positive |
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