Effect of some cultural factors on T1 broth vaccine for contagious bovine pleuropneumonia

The effect of some aspects of buffering capacity, glucose concentration, length of incubation and extent of exposure to air during the preparation of live Mycoplasma mycoides var. mycoides strain T1 broth culture vaccine was investigated. A consistently good vaccine was obtained using 0.07 M phosphate buffered medium containing 0.1% glucose and incubating in stoppered bottles. The shelf-life of this vaccine stored at 4 degrees C was up to 12 weeks. The effect of other storage temperatures on the viability of the vaccine is given.