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人工合成小麦衍生品种川麦47的抗条锈病SSR分子标记定位
引用本文:李俊,魏会廷,胡晓蓉,彭正松,杨武云.人工合成小麦衍生品种川麦47的抗条锈病SSR分子标记定位[J].农业生物技术学报,2007,15(2):318-322.
作者姓名:李俊  魏会廷  胡晓蓉  彭正松  杨武云
作者单位:1. 四川省农业科学院作物研究所,成都,610066
2. 西华师范大学生命科学院,南充,637002
基金项目:国家自然科学基金;国家高技术研究发展计划(863计划);国家科技攻关计划;科技部国际科技合作项目;四川省科技攻关项目
摘    要:条锈病是我国小麦最重要的病害之一,严重威胁小麦生产。川麦47是利用高抗条锈硬粒小麦-节节麦人工合成种基因资源与四川高产小麦绵阳26杂交、有限回交育成的高抗条锈病小麦新品种。为明确川麦47抗条锈性遗传基础,将川麦47分别与高感条锈小麦品种台长29杂交,获得杂交F1、F2群体;对川麦47与台长29构建的F2群体(355株)进行了条锈病抗性鉴定和遗传分析,结果表明,川麦47携带一个显性抗条锈病基因;利用SSR分子标记技术和F2分离群体分组分析法研究表明,该抗条锈病基因位于小麦1B染色体上,与微卫星分子标记Xgwm11、Xgwm18、Xgwm273和Xgwm498紧密连锁,遗传距离分别为2.2CM,2.2CM,4.5CM,3.9CM。川麦47可用于分子标记辅助育种。

关 键 词:小麦  条锈病  微卫星标记  基因定位
文章编号:1006-1304(2007)02-0318-05
收稿时间:2006-08-29
修稿时间:2006-11-15

Molecular Mapping of Stripe Rust Resistance Gene in Synthetic Derivative Chuanmai 47
LI Jun,WEI Hui-ting,HU Xiao-rong,PENG Zheng-song,YANG Wu-yun.Molecular Mapping of Stripe Rust Resistance Gene in Synthetic Derivative Chuanmai 47[J].Journal of Agricultural Biotechnology,2007,15(2):318-322.
Authors:LI Jun  WEI Hui-ting  HU Xiao-rong  PENG Zheng-song  YANG Wu-yun
Institution:1. Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China; 2. Life Science College, China West Normal University, Nanchong 637002, China
Abstract:Stripe rust, caused by Puccinia striiformis f. sp. tritici (PST), is one of the most devastating diseases in common wheat (Triticum aestivum L.) in China. Chuanmai 47, a new wheat variety derived from synthetics (Triticum durum×Aegilops tauschii), was highly resistant to Chinese newly predominant races of Chinese Yellow Race (CYR32) at adult stage. To map the resistant gene in Chuanmai 47, a total of 355 F2 progenies derived from the cross between resistant cultivar chuanmai47 and susceptible variety Taichang29 were used for the resistance gene identifying and genetic analysis. Results indicate that the stripe rust resistance of chuanmai47 conferred by a single dominant gene. And the resistance gene close to the centromere of chromosome 1B and closely linked to the marker Xgwm11, Xgwm498 and Xgwm273 with a genetic distance of 2.2cM, 3.9cM and 4.5cM. Chuanmai47 could be used as strip resistant germplasm in wheat breeding by molecular assistant selection(MAS).
Keywords:wheat  stripe rust  SSR markers  gene mapping
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