| 响应面法优化绣球菌多糖脱色工艺研究 |
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| 引用本文： | 王宏雨,张迪,廖剑华,林衍铨,王泽生. 响应面法优化绣球菌多糖脱色工艺研究[J]. 福建省农科院学报, 2014, 0(1): 67-71 |
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| 作者姓名： | 王宏雨 张迪 廖剑华 林衍铨 王泽生 |
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| 作者单位： | 福建省农业科学院食用菌研究所,福建 福州350014 |
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| 基金项目： | 国家支撑计划课题（2012BAD14B15）;福建省自然基金项目（2010J01100） |
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| 摘 要： | 利用弱酸性离子树脂HZ-830对绣球菌多糖进行脱色，通过Box-Benhnken中心组合试验和响应面分析法，在前期单因素试验基础上，以脱色温度、脱色pH和脱色时间为自变量，脱色率为响应值，将 HZ-830树脂对绣球菌多糖的脱色工艺进行优化。优化后确定的最佳脱色工艺条件为：脱色温度（A ）＝41℃， pH （B）＝8，脱色时间（C）＝3.5 h ，平均脱色率为87.73％。
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| 关 键 词： | 绣球菌 多糖 脱色 树脂 响应面法 |
Optimization of Resin Decoloration Technology of Sparassis crispa Polysaccharide Using Response Surface Methodology |
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| WANG Hong-yu,ZHANG Di,LIAO Jian-hua,LIN Yan-quan,WANG Ze-sheng. Optimization of Resin Decoloration Technology of Sparassis crispa Polysaccharide Using Response Surface Methodology[J]. , 2014, 0(1): 67-71 |
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| Authors: | WANG Hong-yu ZHANG Di LIAO Jian-hua LIN Yan-quan WANG Ze-sheng |
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| Affiliation: | (Institute of Edible Medicinal Fugi , Fujian Academy of Agicultural Sciences, Fuzhou, Fujian 350014, China) |
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| Abstract: | In this paper , response surface methodology was applied to optimize resin decoloration technology of Sparassis crispa polysaccharide by type HZ-830. On the basis of single factor test ,the mathematical regression model was established about the dependent variable （decolorization rate ） and independent variables （decoloration temperature ,pH ,decoloration time） . The results showed that the optimum decolorizing conditions of Sparassis crispa polysaccharide by resin HZ-830 were as follows ：decolorizing temperature 41℃ ,pH8 ,decolorizing time 3.5h .Under this condition ,the decolorizing rate could reach 87.73% . |
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| Keywords: | Sparassis crispa polysaccharide decoloration resin response surface methodology |
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