固相微萃取技术收集水稻挥发物的比较

【目的】探讨用固相微萃取技术（SPME）不同萃取头萃取到的水稻诱导挥发物的异同，为研究植物挥发物提供方法依据。【方法】用茉莉酸甲酯处理水稻，分别用3种萃取头：100 µm聚二甲基硅氧烷（PDMS）、7 µm PDMS和85 µm聚丙烯酸酯（PA）萃取水稻的挥发物。用GC-MS和GC对挥发物进行定性定量分析，比较3种萃取头在萃取水稻挥发物的总量、成份和组成方面的异同，并与动态顶空收集法（DHS）收集的挥发物成份相比较。【结果】在同样条件下，100 µm PDMS萃取的挥发物总量和种类最多，7 µm PDMS最少。SPME法与DHS法获得的挥发物组成类别相似，但有些组分不同。【结论】不同萃取头对水稻挥发物的萃取效率和选择性是不同的。用SPME法研究水稻等植物挥发物，需根据目标组分的性质选择合适的萃取头。SPME法与DHS法在植物挥发物的收集和分析中各有优缺点。

Comparison of Profiling Rice Volatiles by Means of Solid Phase Microextraction

【Objective】The objective of the study is to provide appropriate volatile collection methods of solid phase microextraction(SPME) for analyzing induced volatiles from plants through comparison of difference in rice volatile profiles collected by different fibres.【Method】 Three SPME fibres including 100 μm polydimethylsiloxane(PDMS),7 μm PDMS and 85 μm polyacrylate(PA) were used to extract volatiles emitted from rice plants that were treated with methyl jasmonate.Gas chromatography(GC) and gas chromatography-mass spectrometry(GC-MS) were used to compare the difference in rice induced volatiles in terms of their amount,profile and composition of extracted volatiles.The difference between SPME method and dynamic headspace sampling(DHS) technique was investigated.【Result】 Under the same condition,the maximum amount and variety of volatiles were found in rice volatiles extracted by 100 μm PDMS,the minimum amount and variety by 7 μm PDMS.The composition of volatiles obtained by SPME and DHS was similar,but profile of volatile compounds differed from each other.【Conclusion】 Three fibres showed varied efficiency and selectivity in extracting volatile compounds.SPME fibres should be chosen appropriately according to the property of target compounds in plant volatiles.Both SPME and DHS have their own advantages and disadvantages in plant volatiles extraction and analysis.