大白菜叶色相关性状的QTL定位与分析

 应用已构建了AFLP遗传图谱的大白菜DH群体, 采用多模型QTL作图的方法, 通过色差计法、叶色素测定法和人为目测法3种方法7个指标对叶色相关性状进行QTL定位和分析。结果表明, 在7个连锁群上共检测到18个QTL, 其中色差计法检测到9个QTL, 叶色素测定法检测到8个QTL, 人为目测法仅检测到1个QTL。另外, 估算了单个QTL的贡献率和加性效应, 发现各QTL的加性效应各不相等, 各位点的贡献率在5.5%～15.6%之间。

Mapping and Analysis of QTL Related to Leaf Color in Chinese Cabbage(Brassica rapa L. ssp. pekinensis)

A Chinese cabbage(Brassica rapa L.ssp.pekinensis)double-haploid(DH)population with a reported AFLP genetic map was employed to map quantitative trait loci(QTL)related to leaf color by using MAPQTL 4.0 package.Tristimulus colorimeter,leafy pigment determination and ocular measurement were used to measure the traits related to leaf color.A total of 18 QTL were detected on 7 linkage groups,9,8 and 1 QTL were detected by 3 methods,respectively.These QTL individually explained 5.5% to 15.6% of the phenotypic variation,and there were unequal gene effects on the traits related to leaf color.These mapped QTL could be used to develop a marker assisted selection program for leaf color in Brassica rapa vegetable varieties.