| 转录靶向猪瘟病毒3'-UTR shRNA细胞株的初步建立 |
| |
| 引用本文： | 徐浩,张彦明,冶贵生,郭抗抗.转录靶向猪瘟病毒3''''-UTR shRNA细胞株的初步建立[J].中国预防兽医学报,2007,29(3):163-166. |
| |
| 作者姓名： | 徐浩 张彦明 冶贵生 郭抗抗 |
| |
| 作者单位： | 西北农林科技大学,动物科技学院,陕西,杨凌,712100 |
| |
| 摘 要： | 利用质粒载体在细胞内转录并加工成siRNA的方法，设计3对针对猪瘟病毒3＇-UTR不同位点的干扰片段，分别与干扰载体pGenesil-1连接，转化DH5a，筛选阳性克隆得到重组干扰质粒pGene-1，pGene-2和pGene-3，脂质体介导转染重组干扰质粒于PK-15细胞，G418抗性筛选得到转录靶向猪瘟病毒3＇-UTRshRNA的PK-15细胞株，为今后应用RNAi研究猪瘟病毒Y-UTR调控病毒复制的功能以及抑制猪瘟病毒增殖的研究奠定了基础。
|
| 关 键 词： | 猪瘟病毒 PK-15细胞 |
| 文章编号： | 1008-0589（2007）03-0163-03 |
| 收稿时间： | 2006-04-24 |
| 修稿时间： | 2006年4月24日 |
Establishment of cell lines transcribing shRNA targeted to the 3'-UTR of CSFV |
| |
| XU Hao,ZHANG Yan-ming,YE Gui-sheng,GUO Kang-kang.Establishment of cell lines transcribing shRNA targeted to the 3''''-UTR of CSFV[J].Chinese Journal of Preventive Veterinary Medicine,2007,29(3):163-166. |
| |
| Authors: | XU Hao ZHANG Yan-ming YE Gui-sheng GUO Kang-kang |
| |
| Abstract: | Three pairs of interfering fragments targeted to 3'-UTR of classic swine fever virus(CSFV) were designed and cloned into the plasmid pGenesil-1 respectively,generating recombinant plasmids pGene-1,pGene-2,pGene-3 respectively.These constructs were individually transformed into PK-15 cells by liposome.The cell lines transcribing shRNA for 3'-UTR of CSFV were established by screening positive clones in the presence of G418.The established cell lines could serve as a foundation for further researches on the 3'-UTR of CSFV and the inhibition of virus replication by RNAi. |
| |
| Keywords: | shRNA 3'-UTR G418 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
