| 农杆菌介导蔗糖:蔗糖果糖基转移酶基因转化甘蔗 |
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| 引用本文： | 唐建平,蔡文伟,王俊刚,罗遵喜,张树珍,林海妹.农杆菌介导蔗糖:蔗糖果糖基转移酶基因转化甘蔗[J].分子植物育种,2009,7(3). |
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| 作者姓名： | 唐建平 蔡文伟 王俊刚 罗遵喜 张树珍 林海妹 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海口,571101 |
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| 摘 要： | 甘蔗是高产高蔗糖型植物的代表,把蔗糖:蔗糖果糖基转移酶基因(1-SST)转入甘蔗并使其得到有效的表达,可将甘蔗中的蔗糖转化成果聚糖.本研究以甘蔗为受体,首先接种甘蔗愈伤,培养20 d后即为胚性愈伤组织,用农杆菌菌液浸染转化.研究结果表明,出芽时PPT的最佳筛选浓度为2.0 mg/L,生根时PPT的最佳筛选浓度为5.0 mg/L.共获得42株抗性植株,对抗性植株进行PCR鉴定,其中26株呈阳性,随机选取3株阳性片段进行测序,与目的基因片段一致,初步证明1-SST基因已经转入甘蔗.
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| 关 键 词： | 农杆菌介导 1-SST基因 甘蔗 生物反应器 |
Agrobacterium-Mediated Transformation of Sugarcane for Sucrose:Sucrose 1-Fructosyltransferase Gene |
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| Tang Jianping,Cai Wenwei,Wang Jungang,Luo Zunxi,Zhang Shuzhen,Lin Haimei.Agrobacterium-Mediated Transformation of Sugarcane for Sucrose:Sucrose 1-Fructosyltransferase Gene[J].Molecular Plant Breeding,2009,7(3). |
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| Authors: | Tang Jianping Cai Wenwei Wang Jungang Luo Zunxi Zhang Shuzhen Lin Haimei |
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| Institution: | Tang Jianping Cai Wenwei Wang Jungang Luo Zunxi Zhang Shuzhen Lin Haimei Institute of Tropical Bioscience , Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Ministry of Agriculture,Key Laboratory of Tropical Crop Biotechnology,Haikou,571101 |
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| Abstract: | Sugarcane is a representative of high-yield plant and high sucrose type, it could transform sucrose into fructan by introducing sucrose:sucrose 1-fructosyltransferase gene (1-SST) into sugarcane and to make it expressed efficiently. In this study, we used sugarcane as receptor, first, inoculated sugarcane wound healing, and it would become embryogenic callus after cultured 20 days. then used bacterial liquid of Agrobacterium for transformation. The results showed that, the best screening concentration of PP... |
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| Keywords: | Agrobacterium-mediated 1-SST gene Sugarcane Bioreactor |
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