Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis |
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| Authors: | Alfredo García Remigio Martínez José Manuel Benitez-Medina David Risco Waldo Luis García Joaquín Rey Juan Manuel Alonso Javier Hermoso de Mendoza |
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| Institution: | Departamento de Sanidad Animal, Facultad de Veterinaria de Cáceres, Universidad de Extremadura, 10003 Cáceres, Spain. |
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| Abstract: | Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tuberculosis, Staphylococcus aureus, or Austwickia chelonae was observed. Finally, the RT-PCR assay was used to evaluate clinical samples collected from naturally infected animals with D. congolensis. The results showed that this assay is a fast and reliable method for diagnosing dermatophilosis. |
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| Keywords: | clinical samples Dermatophilus congolensis dermatophilosis diagnosis real-time PCR |
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