利用SSR分子标记鉴定“中丝2号”丝瓜杂交种纯度

为了建立一套丝瓜种子纯度的快速鉴定方法,采用SSR分子标记对丝瓜杂交一代品种“中丝2号”的纯度进行鉴定,利用24对SSR引物对“中丝2号”丝瓜及其亲本的DNA进行扩增。结果表明:从24对引物中筛选出1对特异性引物ZS-SSR24,能够分别在“中丝2号”母本和父本中扩增出特异的2条多态性条带,大小分别为220 bp和250 bp,F_(1)继承双亲的特异性条带,利用此标记能够鉴定出混在F_(1)中的父本或母本。经田间鉴定,供试丝瓜种子纯度为91.3%,这与分子标记的结果完全吻合,故该标记能够用于“中丝2号”丝瓜品种的纯度鉴定。

Seed Purity Test for Luffa Hybrid 'Zhongsi No.2' by SSR Molecular Markers

Abstract: In order to establish a rapid identification method for seed purity of luffa, SSR molecular markers were used to identify the purity of luffa hybrid ''Zhongsi No.2'', and 24 pairs of SSR primers were used to amplify the DNA of luffa ''Zhongsi No.2'' and its parents. The results showed that a pair of specific primers ZS-SSR24 was selected from 24 pairs of primers, and two specific polymorphic bands were amplified from the female and male parents of ''Zhongsi No.2'', the size of which were 220 bp and 250 bp, respectively. F1 inherited the specific bands of parents, and the male or female parents mixed in F1 could be identified by using this marker. Field identification showed that the seed purity of the tested luffa was 91.3%, which was completely consistent with the molecular marker results. Therefore, the marker could be used for the purity identification of ''Zhongsi No.2'' luffa cultivar.