三种笛鲷的野生群体和养殖群体遗传多样性的微卫星分析

摘要：利用微卫星标记技术，采用10对微卫星引物对南海海域红鳍笛鲷、星点笛鲷、紫红笛鲷3种笛鲷鱼的野生种群（HYE、XYE、ZYE）和养殖种群（HYA、XYA、ZYA）进行了遗传多样性分析。10对微卫星引物在6个群体中共检测到78个等位基因，每个位点的等位基因数目在1~8个之间。6个群体的观测杂合度(Ho)为0.2500~1.0000,平均观测杂合度为：ZYE(0.9550)> ZYA(0.8900)，HYE(0.8950)> HYA(0.8400)，XYE(0.8450)>XYA(0.8100) ；平均多态信息含量(PIC) 为0.3648~0.7964，各野生群体均大于养殖群体；三种笛鲷鱼的野生群体和养殖群体遗传距离HYE与HYA，XYE与XYA，ZYE与ZYA分别为0.1029，0.0371，0.0135，基因分化系数分别为0.0371，0.0211，0.0352。群体遗传结构分析结果表明：红鳍笛鲷、星点笛鲷和紫红笛鲷的遗传多样性较丰富，养殖群体的遗传多样性较野生群体均有一定程度的降低，野生群体和养殖群体分化较弱。

Microsatellites Analysis on Genetic Diversities of Three Species from Wild and Cultured Populations of Snappers (Lutjanus)

Abstract: Use microsatellite technology to study Genetic diversity of wild populations (HYE,XYE,ZYE) and cultured Populations(XYA,XYA,ZYA) of Lutjanus erythopterus and Lutjanus stellatus and Lutjanus argentimaculatus from South China sea. The number of total alleles of the ten microsatellite loci is 78, the average number allele of every locus is 1~8. Mean observed heterozygosity (Ho) of six populations is:ZYE(0.9550)>ZYA(0.8900),HYE(0.8950)>HYA (0.8400)，XYE(0.8450)>XYA(0.8100); mean polymorphism information content (PIC) range form 0.3648 to 0.7964, the number of PIC of wild populations is greater than cultured populations. Genetic distances between wild populations and cultured populations of three species in respective snapper is 0.1029，0.0371，0.0135 and coefficients of gene differentiation is 0.0371，0.0211，0.0352. Study of population genetic structure shows that genetic diversity of three species of snappers is abundant, genetic diversity declines in cultured populations. faint genetic Differentiati-on exists between wild populations and cultured populations.