微量元素对籼稻品种愈伤组织诱导和褐化的影响

为了优化籼稻品种的组织培养特性,以N6培养基为基础,分析了微量元素3种不同浓度处理对籼稻品种粤泰B在组织培养中愈伤组织诱导率及褐化率的影响。结果表明:微量元素的浓度对愈伤组织的诱导率影响不大,而对褐化的控制有明显的影响,在1.2倍浓度下愈伤组织的褐化率最低为10.35%,并且愈伤组织致密、状态较好,0.8倍次之,褐化率为26.15%,而一般使用的1.0倍浓度下褐化率为44.55%,褐化情况是最严重的,并且愈伤组织软化,状态很差。

Effects of Trace Elements on the Inducing and Browning of Indica Cultivar Calli

In order to optimize the tissue culture conditions of Indica rice,the effects of trace elements on calli inducing and browning rate of Indica Yuetai B (YTB) were studied based on N_6 culture medium.The results showed that inducing rate of calli wasn't affected significantly by the concentration of minor elements,whereas the browning rate was the strongly affected.The browning rate was the lowest,which was 10.35%,on the 1.2×concentration of trace elements medium.More importantly,the status of calli was compact with good viability.The browning rate of 0.8×concentration was intermediate with 26.15%,whereas that of 1.0×concentration treat was the highest with 44.55%.