Advantages of a new agar medium in the primary isolation of Mycobacterium bovis

Suspect tuberculous lesions from 116 cattle were examined histologically and cultured for Mycobacterium bovis using 5 different media. The media used were: B83, an agar medium incorporating bovine blood and sodium pyruvate; Middlebrook's agar; 2 variations of Stonebrink's medium; L?wenstein-Jensen medium. The B83 medium and a modification of Stonebrink's medium which had a lowered concentration of malachite green were most successful, detecting 95.2% of tuberculous animals when used together. The B83 medium detected isolates approximately 1 week earlier and had more colonies than the Stonebrink's modification. A combination of 2 slopes of B83 and 2 slopes of modified Stonebrink's medium is recommended for routine culture of samples.