转几丁质酶和 β-1,3-葡聚糖酶基因提高棉花对枯萎病和黄萎病的抗性 |
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引用本文: | 程红梅 简桂良 倪万潮 杨红华 王志兴 孙文姬 张保龙 王晓峰 马存 贾士荣. 转几丁质酶和 β-1,3-葡聚糖酶基因提高棉花对枯萎病和黄萎病的抗性[J]. 中国农业科学, 2005, 38(6): 1160-1166 |
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作者姓名: | 程红梅 简桂良 倪万潮 杨红华 王志兴 孙文姬 张保龙 王晓峰 马存 贾士荣 |
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作者单位: | [1]中国农业科学院生物技术研究所,北京100081 [2]中国农业科学院植物保护研究所,北京100094 [3]江苏省农业科学院遗传生理研究所,南京210014 |
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摘 要: | 枯、黄萎病是世界棉花生产中的两大重要病害。传统育种缺乏抗源,几丁质酶和 -1,3-葡聚糖酶是植物防御体系中的两种防卫因子,两者之间存在协同增效作用。据此构建了4个单价和2个双价基因(分别定位于细胞内或细胞外)的植物表达载体,通过花粉管通道法转化棉花,经PCR和Southern杂交检测以及1996 2000年温室及病圃多代筛选鉴定,已培育出对枯、黄萎病抗性提高的转基因棉花株系。将抗病基因导入国产抗虫棉品种GK19中,还获得了兼抗病、虫的转基因优系。
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关 键 词: | 几丁质酶基因 3-葡聚糖酶基因 转基因棉花 枯、黄萎病抗性 |
收稿时间: | 2005-01-17 |
Increase of Fusarium- and Verticillium-Resistance by Transferring Chitinase and Glucanase Gene into Cotton |
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Abstract: | Fusarium and Verticillium wilt are two important diseases in cotton in the world. Traditional breeding method does not have resistance germplasm. Chitinase and -1,3-glucanase are two factors with synogestic effects involved in the plant defense system. Based on this fact, a series of plant expression vectors either harboring chitinase gene or -1,3-glucanase gene or both were constructed. The two genes are expected to be expressed and localized in vacuole or in extra-cellular space. The transformation of cotton was conducted by pollen-tube pathway method. Molecular analysis of transgenic cotton plants by polymerase chain reaction and Southern hybridization, and successive repeated selection of transgenic cotton lines in greenhouse and fungi-infected nurseries during the period of 1996-2000 demonstrated that the transgenic cotton lines with Fusarium wilt and Verticillium wilt resistance were obtained. Meanwhile, the above anti-fungal genes have been integrated into insect-resistant cotton variety GK19 which resulted in the development of transgenic lines resistant to both cotton boll worm as well as Fusarium and Verticillium wilt. |
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Keywords: | Chitinase gene 3-glucanase gene Transgenic cotton Fusarium and Verticillium wilt resistance |
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