6-BA浓度对桔梗胚珠愈伤组织分化率的影响

[目的]研究6-BA浓度对桔梗胚珠愈伤组织分化率的影响.[方法]以桔梗未受精胚珠为外植体,利用N6 ＋1.0 mg/L2,4-D＋0.1 mg/L KT培养基诱导出的愈伤组织作为接种材料,分别接在0.5 mg/L NAA与不同6-BA浓度的分化培养基上,对其愈伤组织分化率进行研究.[结果]6-BA浓度在0.25、1.25和1.00 mg/L（即3种分化培养基为N6＋ 0.5 mg/L NAA＋ 0.25 mg/L 6-BA、N6＋ 0.5 mg/LNAA＋ 1.25 mg/L 6-BA、N6 ＋0.5 mg/L NAA＋1.00 mg/L6-BA）时的分化率高,分别为88.3％、85.0％和80.0％.[结论]6-BA浓度为0.25 mg/L时有利于桔梗未受精胚珠愈伤组织的分化.

Effect of Different Concentration of 6-BA on Differentiation Rate of Ovule Callus of Platycodon Grandiflorum

[ Objective ] To study the effect of different concentration of 6-BA on differentiation rate of ovule callus of Platycodon grandiflorum. [ Methods] Using callus induced from N6 ＋ 1.0 mg/L 2,4-D ＋ 0. 1 mg/L KT medium and inoculated on 0.5 mg/L NAA differentiation media contained different concentration of 6-BA with unfertilized ovtdes of Platycodon grandiflorum as explant. [ Results] The results showed that, the differentiation rate under the differentiation media of N6 ＋0.5 mg/L NAA ＋0.25 mg/L 6-BA, N6 ＋0.5 mg/L NAA ＋ 1.25 mg/L 6-BA and N6 ＋ 0.5 mg/L NAA ＋ 1.00 mg/L 6-BA are higher, up to 88.3%, 85.0%, 80.0% respectively. [ Conclusion ] At 6-BA concentration of 0.25 mg/L, it is favorable for differentiation of unfertilized ovules of Platycodon grandiflorum.