| Abstract: | In the paper, the full length cDNA of RsMF2 gene, homologous with the BcMF2 gene encoding pollen-specific polygalacturonase of Chinese cabbage-pak-choi (Brassica campestris L. ssp. chinensis Makino) was cloned from Raphanus sativus L. cv. Yuanbai by PCR, with a pair of primer designed according to the coding sequence of BcMF2. The largest opening reading frame of RsMF2 gene is 1 266 bp in length and encodes a protein of 421 amino acids with a predicted molecular mass of 43.9 kDa. Sequence analysis revealed that it has three potential N-glycosylation sites and one polygalacturonase active position (RVTCGPGHGLSVGS). And the first 32 amino acids of the predicted RsMF2 protein form a N-terminal hydrophobic domain which displays the properties of a signal peptide. The predicted secondary structure composition for the protein has 6.9% helix, 42.0% sheet and 51.1% loop. Four domains which are highly conserved in the whole plant and fungal PGs is present in RsMF2. Phylogenetic analysis showed that RsMF2 falls into the category of clade-C, which includes PGs related to pollen. These results indicate that RsMF2 may act as polygalacturonase related to pollen development. |
