松油烯-4-醇对家蝇超氧化物歧化酶、过氧化氢酶及过氧化物酶的影响

以杀虫植物砂地柏Sabina vulgaris Ant.精油中主要杀虫成分——松油烯-4-醇点滴处理家蝇成虫后,测定其兴奋期、痉挛期、麻痹期及复苏期等4个中毒阶段试虫的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)及过氧化物酶(POD)活性。结果表明:SOD被明显激活,各中毒阶段的酶比活力均较对照高,差异显著,且表现为先增高后下降的趋势;CAT先被抑制后被激活,在复苏期酶比活力为对照的1.19倍,其中兴奋期和复苏期酶比活力与对照差异显著,而痉挛期和麻痹期与对照无明显差异;POD被明显激活,各中毒阶段的酶比活力依次为对照的2.25、2.04、1.23和2.91倍,与对照差异极显著。上述结果表明,保护酶活性的变化与松油烯-4-醇的作用有密切关系,SOD活力增加可能是松油烯-4-醇的诱导反应,而中毒试虫的复苏可能与CAT和POD活力的变化有关。

The Effect of Terpinen-4-ol on Endogenous Enzymes of Protective System in Housefly

The effects of terpinen-4-ol, a main insecticidal composition in essential oil of (Sabina) vulgaris Ant,on activities of endogenous enzymes of protective system in adult of housefly ((Musca) domestica L.) were studied. The result indicated that superoxide dismutase (SOD) was activated by (terpinen)-4-ol, SOD activities in all poisoning stages were significantly higher than that of the control. The activity of catalase (CAT) was inhibited by terpinen-4-ol in exciting stage, but was gradually activated, it was 1.19 times of control in recovering stage. Peroxidase (POD) was also significantly activated by terpinen-4-ol, the activities were respectively 2.25,(2.04),1.23 and 2.91 times of that of control in exciting stage, convulsing stage, paralysis stage and recovering stage. The activities of (endogenous) (enzymes) of protective system were closely (related) to the effect of terpinen-4-ol. SOD activities were increased, may be due to the in duced reaction of terpinen-4-ol. The recovering of insects poisoned was probably related to the change of POD and CAT activities.