双重抑制PCR技术分离鹅微卫星标记

用双重抑制PCR技术分离11对鹅的特异微卫星引物，应用于浙东白鹅和太湖鹅的基因组PCR扩增。结果表明：11对引物在浙东白鹅和太湖鹅中分别扩增出37和36个清晰条带，20个等位基因为两品种所共有，11对引物中有5对呈中等多态、6对呈高度多态。各位点产生2～6个等位基因，各位点的平均杂合度为0．456～0．772，以G07位点为最高，G06位点为最低；平均多态信息为0．375～0．737，以G07位点为最高，C01、G03和G11位点为最低。浙东白鹅和太湖鹅有效等位基因数分别为2．655和2．558，与实际测得的两品种平均值3．364和3．273较接近。研究结果表明，两品种的等位基因分布较为均匀，与鹅的实际品种特征一致，因此，双重抑制PCR技术适合于鹅的基因组结构研究。

Isolation of goose microsatellite markers using dual-suppression-PCR technique

A dual-suppression-PCR technique was used to isolate 11 goose special microsatellite primers from goose,then those primers were used to amplify DNA of Zhedong White Goose(Z)and Taihu Goose(T).The results showed that 11 primers could generate 37 and 36 clear bands in two breeds,respectively,in which there were 20 same alleles.There were 6 loci with high polymorphism and 5 loci with middle polymorphism.Alleles produced by these loci ranged from 2 to 6 per locus and the mean heterozygosities per locus were calculated as values from 0.456 to 0.772,G07 was the highest and G06 was the lowest and the average polymorphism information content(PIC)per locus were from 0.375 to 0.737,in which microsatellite G07 was the highest,G01,G03 and G11 were the lowest.On the basic of effective number of alleles(Ne),the statistical values of Z and T were 2.655 and 2.558,close to practical average values 3.364 and 3.273.The results indicated that the distributions of alleles in these two breeds were uniform,which were responding to the actual breed characters,and the dual-suppression-PCR technique was suitable for studying goose genetic constructions.