| HAL1基因转化苜蓿再生植株及其耐盐性 |
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| 引用本文： | 刘艳芝,韦正乙,邢少辰,谭化,高明,董英山.HAL1基因转化苜蓿再生植株及其耐盐性[J].吉林农业科学,2008,33(6). |
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| 作者姓名： | 刘艳芝 韦正乙 邢少辰 谭化 高明 董英山 |
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| 作者单位： | 吉林省农业科学院生物技术中心,长春,130033 |
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| 摘 要： | 用根癌农杆菌介导法将克隆于酵母的HAL1基因转化龙牧803苜蓿胚性愈伤组织,经2 mg/L的Basta抗性筛选及PCR检测,该基因已整合到受体基因组中,共获得了11株转基因植株。培养基耐盐性实验结果:在添加NaCl的MSO培养基上,非转基因植株在NaCl浓度高于0.6%时不能生根,逐渐死亡;转基因植株在NaCl浓度0.6%～1.0%范围内仍能生根并正常生长。由此初步证明HAL1基因已在龙牧803苜蓿中表达,并且提高了耐盐性。
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| 关 键 词： | HAL1基因 遗传转化 耐盐性 苜蓿 |
Transgenetic Alfalfa with HAL1 Gene and Its Salt Tolerance |
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| LIU Yan-zhi,WEI Zheng-yi,XING Shao-chen,TAN Hua,GAO Ming,DONG Ying-shan.Transgenetic Alfalfa with HAL1 Gene and Its Salt Tolerance[J].Jilin Agricultural Sciences,2008,33(6). |
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| Authors: | LIU Yan-zhi WEI Zheng-yi XING Shao-chen TAN Hua GAO Ming DONG Ying-shan |
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| Institution: | LIU Yan-zhi,WEI Zheng-yi,XING Shao-chen,TAN Hua,GAO Ming,DONG Ying-shan(Biotechnology Center,Academy of Agricultural Sciences of Jilin Province,Changchun 130033,China) |
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| Abstract: | The HAL1 gene cloned from yeast was introduced into alfalfa cultivar 'Longmu 803' em-bryo cellus.After screened by 2 mg/L of Basta and PCR,the HAL1 gene was proved inserted into the host genome and 11 transgenic plantlets were obtained.A salt-tolerance evaluation was carried out and the re-sult showed that the untransgenic plants could not root on the medium MSO with a concentration of NaCl higher than 0.6% and died gradually.Contrasted with the untransgenic plants,the transgenic plants could root in the me... |
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| Keywords: | HAL1 gene Transformation Salt tolerance Alfalfa |
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