In vitro propagation of spathiphyllum |
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Authors: | Mogens Fonnesbech Anne Fonnesbech |
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Affiliation: | Plant Growth and Development Group, Department of Plant Physiology and Anatomy, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, 1871 Copenhagen V Denmark |
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Abstract: | An in vitro method for propagation of Spathiphyllum cultivar ‘Clevelandii’ is described as an alternative vegetative-propagation method.Leaves, inflorescences, peduncles, buds and stem pieces were tried as explants. Initiation and development of shoots did not occur when leaves and peduncles were used. In a few cases, it was possible to induce shoots from pieces of inflorescence explants. Buds and especially stem pieces were very suitable as explants, with shoots developing on the basal medium both with or without a cytokinin.Further multiplication of the shoots was optimal on the basal medium supplemented with 2 mg/l PBA. On this medium, the basal part of the old shoots became swollen and callus-like, and new shoots emerged from the callused area.Shoots developed roots very easily on the basal medium without hormone additives. |
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