妊娠中期奶山羊下丘脑中催产素的免疫组化定位

采用免疫组织化学技术中的链霉素抗生物素-过氧化酶(streptarid in-perox idase,SP)法,对妊娠中期奶山羊下丘脑中催产素(O xytoc in,OT)神经元的定位及分布进行了观察研究。结果表明,下丘脑中分泌OT的神经元主要分布在视上核和室旁核,在环核、视上弥散核、弓状核、室周核、穹窿周核、下丘脑前区、下丘脑外侧区、下丘脑后核和乳头体各核团等也有一定数量的阳性神经元;另外,在室旁核、视上弥散核、视上核、正中隆起和第三脑室附近有较多数量的强阳性神经纤维,在交叉上核有少量阳性神经纤维。提示OT在妊娠中期奶山羊下丘脑中分布广泛,且下丘脑OT经神经垂体,第三脑室,血管三条途径释放,经血液循环最终作用于靶细胞而发挥生理作用。

Immunohistochemical Localization of Oxytocin on Hypothalamus in Dairy Goat during Midgestation

The immunohistochemical SP method was used to study the distribution of oxvtocin on hypothalamus in dairy goat during midgestation.The oxytocin-immunohistochemical (OT-IR) neurons were mainly distributed over nucleus pareventricularis and nucleus supra-optious:in addition, OT-IR neurons were also found in nucleus circularis, nucleus supra-opticus diffuses, nucleus arcuatus, nucleus periventricularis, nucleus perifornicalis, area anterior hypothalami, area lateralis hypothalami, nucleus posterior hypothalami, nucleus mamilaris and so on.More OT-IR nerve fibers were observed in nucleus paraventricularis, nucleus supra-opticus diffuses, nucleus supra-optious, medium eminence and the surrounding area of the third ventricle and soon.Also, some nerve fibers were found in nucleus suprachiasmaricus.These results suggested that the distribution of OT in the hypothalamus of dairy goat during midgestation was extensive and the physiological effection was on target cells by blood circle after releasing by three channels which were the third ventriculus (cerebrospinal fluid).neurohypophysis and blood vessel.