3 种常用培养基对颠茄毛状根生长与次生代谢产物积累的影响

研究了B5,MS,N6 3种培养基对颠茄毛状根的生长及托品烷类生物碱质量分数和产量的影响.结果表明:B5,MS培养基中颠茄毛状根的生物量明显高于N6培养基,两者之间差异无统计学意义,但B5培养基中东莨菪碱和莨菪碱质量分数与产量均显著高于MS培养基.因此,比较3种培养基,B5培养基作为基本培养基不仅有利于颠茄毛状根的生长,也对托品烷类生物碱的合成有促进作用,为建立适合其生长和次生代谢产物产生的离体培养体系提供了理论依据.

Effects of Three Media on the Growth and Secondary Metabolite Contents of Atropa belladonna L. Hairy Roots

In order to provide a basis for establishing an in vitro culture system suitable for the growth and secondary metabolite production of Atropa belladonna L., a suspension culture was made in which the effects of medium types on the growth of A. belladonna hairy roots and TAs production in them were studied. The biomass of A. belladonna hairy roots in B5 and MS was significantly higher than in N6, with no significant difference between the two media. However, the content and production of scopolamine and hyoscyamine in B5 were significantly higher than in MS.These results showed that B5 was the best medium for hairy root growth of A. belladonna and for the accumulation of tropane alkaloids in it.