| 珠美海棠MzWRKY基因家族盐胁迫应答模式研究 |
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| 引用本文： | 蒋阿维,张素维,孙杨吾,褚欣然,赵宇,许雪峰,孔瑾.珠美海棠MzWRKY基因家族盐胁迫应答模式研究[J].园艺学报,2010,37(8):1213-1219. |
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| 作者姓名： | 蒋阿维 张素维 孙杨吾 褚欣然 赵宇 许雪峰 孔瑾 |
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| 作者单位： | （中国农业大学农学与生物技术学院，北京市果树逆境生理与分子生物学实验室，北京 100193） |
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| 基金项目： | 国家自然科学基金项目，北京市自然科学基金项目，中央高校基本科研业务费专项项目 |
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| 摘 要： | 对苹果属植物现有的58个WRKY 的EST序列进行分析，得到了37条uniESTs。珠美海棠（Malus zumi Mats）是耐盐的优良苹果砧木，能在土壤全盐含量为0.6%的盐碱地上存活。根据已知的37条uniESTs序列，通过半定量RT-PCR研究了珠美海棠中MzWRKY基因家族的盐应答模式。28个MzWRKY基因能检测到RT-PCR的产物，其中21个基因受盐诱导，1个受盐抑制。根据MzWRKY基因盐应答模式可将这些基因分为两组，基因表达模式的差异说明MzWRKY在珠美海棠的盐应答中角色的多样性。
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| 关 键 词： | 珠美海棠 WRKY转录因子 盐胁迫 半定量RT-PCR |
| 收稿时间： | 2010-03-29 |
Expression Analysis of WRKY Family in Response to Salt Stress in Malus zumi Mats |
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| JIANG A-wei,ZHANG Su-wei,SUN Yang-wu,CHU Xin-ran,ZHAO Yu,XU Xue-feng,KONG Jin.Expression Analysis of WRKY Family in Response to Salt Stress in Malus zumi Mats[J].Acta Horticulturae Sinica,2010,37(8):1213-1219. |
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| Authors: | JIANG A-wei ZHANG Su-wei SUN Yang-wu CHU Xin-ran ZHAO Yu XU Xue-feng KONG Jin |
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| Institution: | （Laboratory of Stress Physiology and Molecular Biology for Fruit Tree，College of Agriculture and Biotechnology，China Agricultural University，Beijing 100193，China） |
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| Abstract: | Sequence assembly from 58 Malus WRKY ESTs resulted in 37 uniESTs. Malus zumi Mats is a salt-tolerant rootstock of apple，which could survive in 0.6% total salt content of land. Primers were designed in M. zumi Mats according to the 37 uniESTs，semi-quantitative RT-PCR was applied to reveal the expression patterns of MzWRKY gene family in response to salt stress. RT-PCR products of 28 MzWRKY genes were detectable, 21 MzWRKYs were up-regulated and 1 MzWRKY was down regulated under salt stress. According to the peak hour，the MzWRKYs were classified into 2 groups. The different expression patterns of MzWRKY genes suggested that WRKY proteins play various roles in response to salt stress in M. zumi Mats. |
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