| 三叶草斑潜蝇特异引物的设计与筛选 |
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| 引用本文： | 胡俊韬,吴佳教,李志红. 三叶草斑潜蝇特异引物的设计与筛选[J]. 植物检疫, 2012, 0(3): 36-39 |
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| 作者姓名： | 胡俊韬 吴佳教 李志红 |
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| 作者单位： | 中国农业大学农学与生物技术学院;广东出入境检验检疫局检验检疫技术中心 |
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| 基金项目： | 公益性行业(农业)专项(200903034) |
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| 摘 要： | 斑潜蝇隶属双翅目(Diptera)潜蝇科(Agromyzidae)斑潜蝇属(Liriomyza),是一类重要的农业害虫。近年来,随着海峡两岸果蔬贸易的快速发展,斑潜蝇类害虫传入大陆地区的风险明显增加,植物检疫部门急需该类害虫的快速鉴定技术和方法。本研究针对我国进境检疫性害虫三叶草斑潜蝇(L.trifolii),在检测和检索其线粒体DNA细胞色素氧化酶I(mtDNA COI)基因序列并与其近缘种美洲斑潜蝇(L.sati-vae)和南美斑潜蝇(L.huidobrensis)相同基因序列比对的基础上,设计并筛选了其种类识别特异引物,初步实现了三叶草斑潜蝇的快速鉴定。
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| 关 键 词： | 斑潜蝇 COI基因 特异引物 分子鉴定 |
The designing and selecting of specific primer for molecular identification of Liriomyza trifolii(Burgess) |
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| Hu Juntao,Wu Jiajiao,Li Zhihong. The designing and selecting of specific primer for molecular identification of Liriomyza trifolii(Burgess)[J]. Plant Quarantine, 2012, 0(3): 36-39 |
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| Authors: | Hu Juntao Wu Jiajiao Li Zhihong |
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| Affiliation: | 1*(1.College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China;2.Inspection and Quarantine Technology Center,Guangdong Entry-Exit Inspection and Quarantine Bureau) |
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| Abstract: | Liriomyza is a kind of agricultural insect pest which belongs to Diptera,Agromyzidae.With rapid development of vegetable and flower trades between Taiwan and mainland,the entry risk of Liriomyza increases obviously.The rapid methods and techniques of quarantine identification for Liriomyza are needed urgently at ports.A specific primer pair of L.trifolii(Burgess),the quarantine pest of Liriomyza,were studied in this study.Basing on testing,collecting and comparing the sequences of mitochondrial cytochrome c oxidase subunit I(mtDNA COI) of L.trifolii(Burgess),L.sativae Blanchard and L.huidobrensis(Blanchard),the proper specific primer pair was designed and selected which could accomplish the rapid identification of L.trifolii(Burgess) initially. |
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| Keywords: | Liriomyza COI gene specific primer molecular identification |
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