IP_3敏感的钙离子通透性通道参与茉莉酸诱导的钙动员

目的]研究茉莉酸诱导的钙动员通往IP3敏感的钙离子通透性通道的作用。方法]以低温导入法将钙离子荧光探针Fluo-3/AM导入拟南芥叶片细胞中,利用LASAF（Leica Application Suite-Advanced Fluorescence）软件记录肝素对茉莉酸（JA）诱导的胞内钙离子荧光强度的变化。结果]经不同浓度的肝素预处理后,拟南芥叶细胞中胞内钙离子的荧光强度降低,再用100μmol/LJA处理时,其荧光强度升高,但仅与未经肝素处理的荧光强度相当。结论]肝素预处理可抑制JA诱导的胞内钙离子浓度的升高。

IP3 Sensitive Calcium Channel Involved in the Jasmonic Acid Induced Calcium Mobilization

Objective] This study was to investigate the role of IP3 sensitive calcium channel in the JA-induced calcium mobilization pathway.Method] Arabidopsis thaliana leaves were labeled by Fluorescent probe Fluo-3/AM under low temperature at 4 ℃ to measure the fluorescent intensity of intracellular Ca2+ which was pretreated with heparin on jasmonic acid(JA)-induced.Results] When A.thaliana leaf cells were pretreated with 10,50 or 100 ng/ml heparin,intercellular free Ca2+ fluorescence intensity was reduced in comparison with negative control.Once the heparin-pretreated A.thaliana leaf cells were stimulated with 100 μmol/L JA,intercellular Ca2+ fluorescence intensity increased gradually and tended to be stable at a degree equivalent with that in negative control.Conclusion] The experiment showed that the pretreatment with heparin could inhibit the increase of the intracellular Ca2+ concentration significantly which JA-induced in leaves of Arabidopsis thaliana.