Preparation and characterization of neutralizing monoclonal antibodies to feline calicivirus. |
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| Authors: | Y Tohya Y Taniguchi M Tsubakimoto E Takahashi T Mikami |
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| Affiliation: | Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan. |
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| Abstract: | Fourteen neutralizing monoclonal antibodies (N-MoAbs) were prepared against the F4 strain of feline calicivirus (FCV), the prototype strain of FCV in Japan, and examined for their ability to neutralize FCV isolates. Neutralization-resistant variants of the F4 strain were selected under the presence of 4 individual N-MoAbs in cell culture systems and used in cross-neutralization tests and enzyme-linked immunosorbent assay with all of the 14 N-MoAbs. The results revealed the identification of at least two antigenic determinants on FCV F4: one being more broadly conserved among FCV isolates than the other. Usefulness of antigenic variants resistant to N-MoAbs for analysis of neutralization determinants on FCV was also demonstrated. |
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