| 马铃薯花药培养硝酸银对诱导双单倍体及一单倍体的效果 |
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| 引用本文： | 冉毅东,戴朝曦.马铃薯花药培养硝酸银对诱导双单倍体及一单倍体的效果[J].西北农业学报,1993,2(4):43-47. |
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| 作者姓名： | 冉毅东 戴朝曦 |
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| 作者单位： | 甘肃农业大学农业生物工程研究所, 兰州 730070;甘肃农业大学农业生物工程研究所, 兰州 730070 |
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| 基金项目： | 农业部“八五”生物技术计划;甘肃省青年基金 |
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| 摘 要： | 通过在培养基中加入不同浓度AgNO3,对离体条件下诱导马铃薯四倍体(S.tuberosum.L.)花药产生双单倍体(dihaploid),双单倍体花药产生一单倍体(monohaploid)的效应进行了研究.在基本诱导培养基中加入50～100μmol/LAgNO3可显著促进四倍体花药和双单倍体花药产生胚状体,胚状体出现的频率最高可达16%.同时可提高胚状体的分化率.AgNO3还可延缓花药的褐化,从而延长胚状体出现的时间.
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| 关 键 词： | 花药培养 双单倍体 单倍体 马铃薯 |
| 收稿时间： | 1993/5/3 0:00:00 |
STUDYING ON THE EFFECTION OF AgNO3 ON INDUCING DIHAPLOID AND MONOPLOID IN VITRO BY ANTHER CULTURE IN CULTIVATED POTATO |
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| Ran Yidong and Dai Chaoxi.STUDYING ON THE EFFECTION OF AgNO3 ON INDUCING DIHAPLOID AND MONOPLOID IN VITRO BY ANTHER CULTURE IN CULTIVATED POTATO[J].Acta Agriculturae Boreali-occidentalis Sinica,1993,2(4):43-47. |
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| Authors: | Ran Yidong and Dai Chaoxi |
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| Institution: | Agriculture institute of biotechnology, Gansu agricultural university, Lanzhou 730070;Agriculture institute of biotechnology, Gansu agricultural university, Lanzhou 730070 |
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| Abstract: | The effection of inducing dihaploid from tetraploid and monoploid from dihaploid in potato (Solanum tuberosum L.) in vitro by adding AgNO3 in anther culture medium was studied.Embryoids produced from the anthers of tetraploid and dihaploid were significantly promoted by plusing 50-100 pmol/L AgNO3 in general induce medium,the highest frequency of embryoiods is up to 16%.The Percentage of differentiation of the embryoiods was also arised,AgNO3 could delay anthers turning brown and prolong the emergence period of embryoiods. |
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| Keywords: | AgNO3 Anther culture Potato Dihaploid Monoploid |
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