2013－2019年广西边境地区低致病性禽流感病毒监测分析

为了解广西边境地区低致病性禽流感病毒（low pathogenic Avian influenza virus，LPAIV）的流行情况，本研究对2013-2019年在广西边境地区的规模养殖场和活禽市场采集的33 964份家禽棉拭子样品，采用鸡胚接种方法进行病毒分离，并结合血凝抑制试验和RT-PCR等方法鉴定禽流感病毒的亚型。结果显示，共有3 892份样品分离到LPAIV，2013-2019年的样品病毒分离率分别为17.39%、11.23%、13.59%、9.31%、9.09%、9.12%和15.21%，总分离率为11.46%，其中2013年样品的病毒分离率最高。分离到的LPAIV包括H1、H3、H4、H6、H9、H10和H11 7种亚型，样品病毒分离率分别为0.14%、4.37%、0.19%、4.06%、2.69%、0.01%和0.003%。其中活禽市场分离到H1、H3、H4、H6、H9、H10和H11 7种亚型流感病毒，样品病毒分离率分别为0.19%、5.53%、0.21%、5.29%、3.41%、0.01%和0.004%，总分离率为14.64%；而养殖场分离到H3、H4、H6和H9 4种亚型流感病毒，样品病毒分离率分别为1.26%、0.12%、0.79%和0.78%，总分离率为2.95%，养殖场的分离率远低于活禽市场（14.64%）。鸡源样品的病毒分离率为5.77%，水禽样品的病毒分离率为15.10%，环境样品的病毒分离率为21.67%，水禽和环境样品的病毒分离率远高于鸡。结果表明，当前广西边境地区家禽携带多种亚型LPAIV，水禽是高风险禽群，应进一步加强监测和防控；活禽市场污染严重，应进一步加强对活禽市场的监管力度，建立和实施更加科学有效的活禽市场运营机制。

Surveillance Analysis of Low Pathogenic Avian Influenza Virus in Border Areas of Guangxi from 2013 to 2019

In order to understand the low pathogenic Avian influenza virus (LPAIV) epidemic situation in Guangxi border areas from 2013 to 2019, 33 964 poultry swabs samples were collected from large-scale farms and live poultry markets for LPAIV subtype isolation and identification through hemagglutination inhibition test and RT-PCR.The results showed that 3 892 LPAIV strains were isolated, the virus isolation rates from 2013 to 2019 were 17.39%, 11.23%, 13.59%, 9.31%, 9.09%, 9.12% and 15.21%, and the total isolation rate was 11.46%, of which the rate in 2013 was the highest.The isolated LPAIV included 7 subtypes H1, H3, H4, H6, H9, H10 and H11.These virus isolation rates were 0.14%, 4.37%, 0.19%, 4.06%, 2.69%, 0.01% and 0.003%.In the live poultry market, LPAIV subtype H1, H3, H4, H6, H9, H10 and H11 rates were 0.19%, 5.53%, 0.21%, 5.29%, 3.41%, 0.01% and 0.004%, respectively.While in the farms LPAIV subtype H3, H4, H6 and H9 rates were 1.26%, 0.12%, 0.79% and 0.78%, respectively.The virus isolation rate of the farm samples (2.95%) was obviously lower than the live poultry market samples (14.64%).The virus isolation rates of waterfowl (15.10%) and environmental samples (21.67%) were much higher than that from chickens (5.77%).The results showed that the current poultry carried multiple LPAIV subtypes, and waterfowl were high-risk flocks.The live poultry market was seriously polluted, and the market supervision (LPAIV) should be further strengthened, established and implemented more scientific, effective live poultry market operation mechanism.