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| 我国片形吸虫线粒体NADH脱氢酶亚单位1基因(nad1)多态性的研究 | |
| 引用本文： | 黄维义,董世娟,杨晓野,林瑞庆,钱德兴,张为宇,宋慧群,朱兴全.我国片形吸虫线粒体NADH脱氢酶亚单位1基因(nad1)多态性的研究[J].中国预防兽医学报,2005,27(4):273-276. |
| 作者姓名： | 黄维义 董世娟 杨晓野 林瑞庆 钱德兴 张为宇 宋慧群 朱兴全 |
| 作者单位： | 1. 广西大学,动物科学技术学院,广西,南宁,530005 2. 广西大学,动物科学技术学院,广西,南宁,530005;华南农业大学,兽医学院,广东,广州,510642 3. 内蒙古农业大学,动物科学与医学学院,内蒙古,呼和浩特,010018 4. 华南农业大学,兽医学院,广东,广州,510642 5. 贵州省兽医防疫检疫总站,贵州,贵阳,550008 |
| 基金项目： | 国家杰出青年科学基金项目(No.30225033)，国家自然科学基金项目(No.30260082)，华南农业大学校长基金 |
| 摘 要： | 用γ^33P对引物进行标记，首次对来自国内不同地区、不同宿主的片形吸虫线粒体烟酰胺腺嘌呤二核苷酸(NADH)脱氢酶亚单位Ⅰ基因部分序列(pnad1)进行PCR扩增及DNA单链构象多态性(PCR-SSCP)分析，结果76个虫体均成功地扩增出约200bp的基因片段；76个样品经过PCR-SSCP分析后，筛选出11个代表性样品进行测序。测序结果显示我国片形吸虫pnad1序列种间差异大于种内变异，表明nad1序列可以作为片形吸虫种内和种间遗传多态性研究的标记。 |
| 关 键 词： | 片型吸虫 nad1 PCR-SSCP 多态性 |
| 文章编号： | 1008-0589(2005)04-0273-04 |
| 修稿时间： | 2004年1月12日 |
DNA polymorphism of the mitochondrial NADH dehydrogenase subunit 1 gene(nad 1)of fasciola from China |
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| HUANG Wei-yi,DONG Shi-juan,YANG Xiao-ye,LIN Rui-qing,QIAN De-xing,ZHANG Wei-yu,SONG Hui-Qun,ZHU Xing-quan.DNA polymorphism of the mitochondrial NADH dehydrogenase subunit 1 gene(nad 1)of fasciola from China[J].Chinese Journal of Preventive Veterinary Medicine,2005,27(4):273-276. | |
| Authors: | HUANG Wei-yi DONG Shi-juan YANG Xiao-ye LIN Rui-qing QIAN De-xing ZHANG Wei-yu SONG Hui-Qun ZHU Xing-quan |
| Abstract: | A portion (approximately 200 bp in length) of the mitochondrial dehydrogenase (NADH)subunit 1 gene(pnad 1)was amplified from 76 specimens of Fasciola from different geographical locations and different hosts in mainland China by PCR with 33P_endlabelled primers, and the amplicons were analysed by single_strand conformation polymorphism(SSCP).11 representative specimens were selected and sequenced. Sequence analyses revealed that interspecific sequence difference in pnad1 was significantly higher than intraspecific variation among specimens of Fasciola from Mainland China.The nad1 sequence could be used as genetic marker for studying the classification and systematics of Fasciola species. |
| Keywords: | Fasciola nad1 PCR_SSCP polymorphism |
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