4个动物园灵长类动物肠道克雷伯氏菌的分离鉴定与药敏试验

从4个动物园的非人灵长类动物中采集了214份粪便样品,经过细菌分离培养、染色镜检和生化试验等分离鉴定出88株克雷伯氏菌,分离率为41.12%。按照美国临床实验室标准化委员会(CLSI)的判定标准,对分离到的88株克雷伯氏菌进行了22种抗生素药物的药敏试验,结果表明,88株均表现出不同的程度的耐药性,其中,耐药程度最高的是羧苄青霉素(耐药率为95.45%);敏感度最高的是阿米卡星(敏感率为77.27%)。88株克雷伯氏菌分离株均有较为严重的多重耐药性,耐8～15种抗菌药物的菌株最多,有64株,占分离菌株的72.73%;耐10种以上抗菌药物的菌株有63株,占分离菌株的71.59%。从不同动物园分离的克雷伯氏菌对22种抗生素的耐药情况来看,在所调查的4个动物园中,重庆某动物园(CQ)和北京某动物园(BJ)的克雷伯氏菌分离株间耐药率无差异(P>0.05);上海某野生动物园(SHW)和上海某动物园(SHZ)的克雷伯氏菌分离株间耐药率无差异(P>0.05);而北京某动物园(BJ)与上海某野生动物园(SHW)和上海某动物园(SHZ)的克雷伯氏菌分离株相比,其耐药率差异均极显著(P<0.01);重庆某动物园(CQ)与上海某野生动物园(SHW)和上海某动物园(SHZ)的克雷伯氏菌分离株相比,其耐药率差异均极显著(P<0.01)。

Isolation,Identification and Antibiotics Sensitivity of Non-human Primates Klebsiella in Four Zoogical Gardens

A total of 214 fecal samples were collected and examined for intestinal Klebsiella. 88 strains of Klebsiella were isolated and identified by a series of tests, including isolation of bacteria, biochemical tests, etc. The separation rate was 41.12%. According to the clinical and laboratory standards institute (CLSI), antimicrobial susceptibility test, against 22 kinds of common antibiotics and other antibiotics, was carried out for the 88 strains of Klebsiella. Our results indicated that the 88 strains of Klebsiella showed varying degrees of drug resistance. They were of the highest resistance to carbenicillin (drug resistance rate was 95.45%). They had the highest sensitivity to amikacin (sensitivity rate was 77.27%).The phenomenon of multiple drug resistance occurred in all strains of Klebsiella. 64 of them were indentified to be resistant to 8 to 15 kinds of antibiotics,63 of them were resistant to more than ten kinds of antibiotics. On the other hand, the differences of drug resistance rates of Klebsiella against 22 kinds of common antibiotics and other antibiotics in four zoological gardens were considered. There was no significant difference of drug resistance rates of Klebsiella collected in CQ and BJ(P>0.05). The same thing occurred in SHW and SHZ, the strains of Klebsiella collected from the two zoological gardens performed the similar drug resistance rates. However, the drug resistance rates of Klebsiella in both SHW and SHZ extremly significantly differed from either BJ or CQ(P<0.01).