| 水产品中替米考星残留量检测的条件优化 |
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| 引用本文： | 苏秀华,吴成业,钱卓真. 水产品中替米考星残留量检测的条件优化[J]. 南方水产, 2010, 6(3): 12-18. DOI: 10.3969/j.issn.1673-2227.2010.03.003 |
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| 作者姓名： | 苏秀华 吴成业 钱卓真 |
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| 作者单位： | 1. 福建农林大学,福建,福州,350002;福建省水产研究所,福建,厦门,361012
2. 福建省水产研究所,福建,厦门,361012 |
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| 基金项目： | 福建省海洋与渔业厅重点项目 |
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| 摘 要： | 文章探讨了用高效液相色谱（HPLC）测定水产品中替米考星残留量的方法。通过对分析条件的优化，确定了最佳检测参数，并从提取和净化2个方面确立了前处理的最佳步骤与条件。通过验证不同基质试样的加标回收试验，结果显示，在20～200μg·kg-1的添加水平下回收率为77．22％～93．25％，相对标准偏差（RSD）小于5％，定量检出限（LOQ）为20μg·kg-1。分析结果表明，所建立的方法灵敏度高、准确性好且应用范围广，可用于日常水产品中替米考星残留量的检测分析。
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| 关 键 词： | 水产品 替米考星 残留 高效液相色谱 |
Study on determination of Tilmicosin residue in aquatic products |
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| SU Xiuhua,WU Chengye,QIAN Zhuozhen. Study on determination of Tilmicosin residue in aquatic products[J]. South China Fisheries Science, 2010, 6(3): 12-18. DOI: 10.3969/j.issn.1673-2227.2010.03.003 |
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| Authors: | SU Xiuhua WU Chengye QIAN Zhuozhen |
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| Affiliation: | 1. Fujian Agriculture and Forestry University, Fuzhou 350002, China ; 2. Fisheries Research Institute of Fujian, Xiamen 361012, China) |
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| Abstract: | A HPLC method for determining Tilmicosin in aquatic products was discussed in this paper. The optimal detection parame- ters were determined by optimizing the analytical conditions ; the best procedure and factors of pretreatment were determined in the as- pects of extraction and clean-up. Through the experimental verification of different sample substrates, the recovery of standard addition reached 77.22% - 93.25 % and RSD was less than 5 % with the addition of 20 - 200 μg·kg-1 Moreover, the limit of quantitation was 20μg·kg-1. The results showed that this method had advantages of high test sensitivity, accuracy and wide range of application. It could be used for routine detection and analysis of Tilmicosin residue in aquatic products. |
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| Keywords: | aquatic products Tilmicosin residue HPLC |
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