| 巴西橡胶树半胱氨酸蛋白酶基因(HbCP1)的原核表达分析 |
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| 引用本文： | 朱家红,李辉亮,彭世清. 巴西橡胶树半胱氨酸蛋白酶基因(HbCP1)的原核表达分析[J]. 热带作物学报, 2009, 30(5): 667-671 |
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| 作者姓名： | 朱家红 李辉亮 彭世清 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海口,571101;中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海口,571101;中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海口,571101 |
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| 基金项目： | 中央级公益性科研院所基本科研业务专项基金 |
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| 摘 要： | 为研究巴西橡胶树半胱氨酸蛋白酶生物学功能,利用笔者从橡胶树中克隆的半胱氨酸蛋白酶基因(HbCPI)(GenBank登录号:DQ642886)序列,构建了HbCP1基因的原核表达载体,并在大肠杆菌中诱导表达.结果表明,HbCP1基因在大肠杆菌中的表达产物具有生理毒性,抑制大肠杆菌的生长,初步确认RTX结构域是导致HbCPI表达产物具有生理毒性的原因.
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| 关 键 词： | 巴西橡胶树 半胱氨酸蛋白酶 HbCP1基因 原核表达 |
Expression Analysis of the Cysteine Protease Gene (HbCP1) in E. coli |
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| Zhu Jiahong,Li Huiliang and Peng Shiqing. Expression Analysis of the Cysteine Protease Gene (HbCP1) in E. coli[J]. Chinese Journal of Tropical Crops, 2009, 30(5): 667-671 |
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| Authors: | Zhu Jiahong Li Huiliang Peng Shiqing |
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| Affiliation: | Zhu Jiahong,Li Huiliang,Peng Shiqing Ministry of Agriculture Key Biotechnology Laboratory for Tropical Crops , Institute of Tropical Biosciences , Biotechnology,CATAS,Haikou 571101,China |
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| Abstract: | Cysteine proteases can be found in the animal and plant kingdoms as well as in some viruses and bacteria. They have been applied in many and diverse aspects of physiology and development. In plants, cysteine proteases play an essential role in development, e.g. embryogenesis, tracheary element differentiation, development and germination of seeds, leaf and flower senescence and in response to biotic and abiotic stresses. The expression vectors pET-30a-HbCP1 and pET-30a-EHbCP1 were constructed and expressed ... |
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| Keywords: | Hevea brasiliensis cysteine proteases HbCP1 prokaryotic expression |
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