| 利用农杆菌介导的共转化法获得转基因水稻 |
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| 引用本文： | 刘永巍,田红刚,孟巧霞,孟昭河,李春光,张景龙. 利用农杆菌介导的共转化法获得转基因水稻[J]. 黑龙江农业科学, 2008, 0(3): 5-7 |
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| 作者姓名： | 刘永巍 田红刚 孟巧霞 孟昭河 李春光 张景龙 |
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| 作者单位： | 黑龙江省农垦科学院水稻研究所,佳木斯,154025 |
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| 基金项目： | 国家高技术研究发展计划(863计划) , 黑龙江省农垦总局科研项目 |
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| 摘 要： | 以水稻粳稻品种空育131、垦稻12为材料,用农杆菌共转化的方法将质粒p1300和p13W8导入受体材料。结果显示：两种混合共转化菌液按体积比1∶1时共转化效率最高,植株转化效率达4.08%和2.63%。将转化植株进行PCR鉴定,13株转基因植株均能检测到质粒p1300的信号,4株检测到质粒p13W8的信号,反义蜡质基因的阳性株占转基因植株总数的30.77%。
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| 关 键 词： | 水稻 农杆菌介导 反义蜡质基因 共转化 |
| 文章编号： | 1002-2767(2008)03-0005-03 |
| 修稿时间： | 2007-12-26 |
Transgenic Rice Plants Obtained by Agrobacterium-mediated Co-transformation Method |
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| LIU Yong-wei,TIAN Hong-gang,MENG Qiao-xia,MENG Zhao-he,LI Chun-guang,ZHANG Jing-long. Transgenic Rice Plants Obtained by Agrobacterium-mediated Co-transformation Method[J]. Heilongjiang Agricultural Science, 2008, 0(3): 5-7 |
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| Authors: | LIU Yong-wei TIAN Hong-gang MENG Qiao-xia MENG Zhao-he LI Chun-guang ZHANG Jing-long |
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| Affiliation: | (Rice Research Institute of Land Reclamation Academy of Heilongjiang Province, Jiamus 154025) |
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| Abstract: | Both of the plasmid p1300 and plasmid p13W8 were introduced into Japonica Rice Kongyu 131 by Agrobacterium-mediated co-transformation,the same two plasmid were introduced into Kendao 12 by the same way. The results showed that the co-transformation efficiency was highest when two kinds of Agrobacterium was 1 ： 1 volume,and the co-transformation efficiency were 4.08% and 2. 63%, respectively. The transformed plants were screened by PCR method,specific fragments of plasmid p1300 could be screened in 13 transgenic lines,specific fragments of plasmid p13W8 could be screened in 4 transgenic lines. Positive Antisense-Waxy Gene plants were 30.77% of all transformed plants. |
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| Keywords: | rice agrobacterium-mediated transformation Antisense-Waxy Gene co-transformation |
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