| 从云南蝴蝶兰上检测到番茄斑萎病毒属病毒 |
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| 引用本文： | 程晓非,董家红,方琦,李婷婷,丁铭,张仲凯.从云南蝴蝶兰上检测到番茄斑萎病毒属病毒[J].植物病理学报,2008,38(1):31-34. |
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| 作者姓名： | 程晓非 董家红 方琦 李婷婷 丁铭 张仲凯 |
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| 作者单位： | 云南省农业生物技术重点实验室 昆明 650223 |
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| 摘 要： | 应用电镜观察、DAS-ELISA以及RT-PCR检测,从症状表现黄化、环斑的云南蝴蝶兰(Phalaenopsis amabilis)病样中分离得到的一个病毒分离物Tospo-Pha。该分离物粒子近球形、具包膜、直径约90nm,与番茄斑萎病毒属(Tospovirus)的西瓜银色斑驳病毒(Watermelon silver mosaic virus,WSMoV)/花生芽坏死病毒(Groundnut bud necrosis virus,GBNV)复合抗血清呈强阳性反应,分子检测发现该分离物SRNA5'末端序列与CaCV大岩桐分离物(CaCV-Gloxinia)同源性最高(91.0%),在系统进化树中与CaCV聚于同一分支。上述结果表明,从云南蝴蝶兰中分离到的Tospo-Pha属于Tospovirus病毒。
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| 关 键 词： | 番茄斑萎病毒属 蝴蝶兰 黄化环斑 |
Detection of a tospovirus infecting Phalaenopsis amabilis in Yunnan |
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| CHENG Xiao-fei,DONG Jia-hong,FANG Qi,LI Ting-ting,DING Ming,ZHANG Zhong-kai.Detection of a tospovirus infecting Phalaenopsis amabilis in Yunnan[J].Acta Phytopathologica Sinica,2008,38(1):31-34. |
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| Authors: | CHENG Xiao-fei DONG Jia-hong FANG Qi LI Ting-ting DING Ming ZHANG Zhong-kai |
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| Institution: | Yunnan Provincial Key Laboratory of Agricultural Biotechnology, Kunming 650223, China |
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| Abstract: | Using electron microscopy,DAS-ELISA and RT-PCR,a virus isolate(Tospo-Pha) was detected from Phalaenopsis amabilis showing yellow ringspot symptoms in Yunnan.Tospo-Pha had quasi-spherical,enveloped shape with diameters of 90 nm,reacted positively to compound antibody of Watermelon silver mosaic virus(WSMoV)/Groundnut bud necrosis virus(GBNV).The S RNA 5'-termini sequence of Tospo-Pha had highest identity(91.0%) with that of CaCV isolate Gloxinia(CaCV-Gloxinia).The Tospo-Pha clustered with other CaCV isolates in phylogenetic tree.These results indicated Tospo-Pha belonged to the genus Tospovirus. |
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| Keywords: | Tospovirus Phalaenopsis amabilis yellow ringspot |
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