| 广西姜花花粉离体萌发培养基的筛选 |
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| 引用本文： | 董芬丽,吴志,胡秀. 广西姜花花粉离体萌发培养基的筛选[J]. 安徽农业科学, 2009, 37(31) |
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| 作者姓名： | 董芬丽 吴志 胡秀 |
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| 作者单位： | 中国科学院华南植物园,广东广州510650;仲恺农业工程学院,广东广州,510225;中国科学院研究生院,北京,100081;仲恺农业工程学院,广东广州,510225 |
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| 摘 要： | [目的]为广西姜花花粉离体萌发筛选出最适培养基。[方法]以原位萌发测定为对照,在正交预试验的基础上,通过单因素试验筛选出广西姜花花粉离体萌发的最适培养基。[结果]蔗糖浓度为10.000%时,广西姜花花粉的离体萌发率最高,为22.45%。当硼酸浓度为0.010%和0.050%时,广西姜花花粉的离体萌发率分别为35.58%和37.42%。当氯化钙的浓度为0.010%时,广西姜花花粉的离体萌发率为38.49%。在0.010%～0.200%浓度范围内,广西姜花花粉的离体萌发率随着氯化钙浓度的升高而下降。广西姜花花粉离体萌发的最适培养基为:10.000%蔗糖+0.050%硼酸+0.010%氯化钙+2.000%琼脂,pH=7.0,培养温度为20℃。在筛选出的培养基上,通过花粉离体萌发测定的花粉萌发率为46.00%。采用花粉原位萌发测定法测定的花粉萌发率为50.00%。[结论]利用筛选出的培养基能够快速准确测定出广西姜花的花粉活力。
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| 关 键 词： | 广西姜花 花粉 离体萌发 活力测定 |
Medium Selection for the Pollen Germination in Vitro of Hedychium kwangsiense T.L.Wu & S.J.Chen |
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| DONG Fen-li et al. Medium Selection for the Pollen Germination in Vitro of Hedychium kwangsiense T.L.Wu & S.J.Chen[J]. Journal of Anhui Agricultural Sciences, 2009, 37(31) |
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| Authors: | DONG Fen-li et al |
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| Affiliation: | DONG Fen-li et al(South China Botanical Garden,Chinese Academy of Science,Guangzhou,Guangdong 510650) |
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| Abstract: | [Objective] The aim was to screen out the optimum medium for the pollen germination in vitro of Hedychium kwangsiense T.L.Wu &S.J.Chen.[Method] With situ-germination determination as CK,on the basis of orthogonal pre-experiment,the optimum medium for the pollen germination in vitro of H.kwangsiense was screened out through single factor experiments.[Result] When the sucrose concn.was 10.000%,the germination rate in vitro of H.kwangsiense pollens was highest,being 22.45%.When the concn.of boric acid was 0.01... |
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| Keywords: | Hedychium kwangsiense T.L.Wu & S.J.Chen Pollen Germination in vitro Test on vitality |
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