解淀粉芽孢杆菌LJ02对黄瓜抗灰霉病菌的生防效果及其诱导抗性机理的初步研究

 为了解生防解淀粉芽孢杆菌LJ02诱导黄瓜抗灰霉病菌的防治效果和作用机制,分别测量了LJ02的发酵上清液和菌体悬浮液的诱导持效期、最适使用浓度以及最佳施用方法,并采用荧光定量PCR的方法法测定了LJ02诱导处理后黄瓜根部和叶部组织中分别表达PR1a蛋白、β-1,3-葡聚糖酶、几丁质酶、过氧化物酶的抗性基因PR-1、PR-2、PR-3、PR-9的相对表达量。结果表明,LJ02诱导黄瓜抗灰霉病菌的持效期在7 d左右,且原液与100倍稀释液效果最好,灌根施用效果最佳。LJ02发酵上清液可诱导PR-1、PR-3、PR-9的表达,LJ02菌体悬浮液可诱导PR-1、PR-2、PR-3的表达。

Preliminary study on the effect of the induced resistance in cucumber with Bacillus amyloliquefaciens LJ02 against Botrytis cinerea

For elucidating the control efficacy and mechanism of cucumber-resistant gray mold induced by Bacillus amyloliquefaciens LJ02, the induction duration, optimum concentration and optimal application method of fermentation broth (FB) and cell suspension (CS) of LJ02 were measured. The relative expression levels of resistance-related genes including PR-1, PR-2, PR-3 and PR-9 (encoding proteins PR1a, β-1,3-glucanase, chitinase and peroxidase, respectively) in cucumber root and leaf tissues after treated with LJ02 were determined by real-time PCR The results showed that the duration of LJ02-induced resistance to gray mold in cucumber was about 7 d with the stock solution and 100-fold dilution by the root irrigation. The LJ02FB can induce the expression of PR-1, PR-3 and PR-9, while the LJ02CS for theexpression of PR-1, PR-2 and PR-3.