‘阳光玫瑰’葡萄病毒小RNA测序鉴定及RT-PCR检测

 ‘阳光玫瑰’是我国从日本引进的葡萄优良品种。为了明确我国‘阳光玫瑰’葡萄病毒病的病原,本研究采用小RNA测序技术对2株显症和无症状的‘阳光玫瑰’葡萄样品进行病毒鉴定结果显示:显症样品中测定到8种病毒,其中包含葡萄蚕豆萎蔫病毒(Grapevine fabavirus, GFabV)和灰比诺葡萄病毒(Grapevine Pinot gris virus, GPGV);无症状样品中测定到3种葡萄病毒。对46个‘阳光玫瑰’样品进行14种葡萄病毒的RT-PCR检测,结果表明:‘阳光玫瑰’葡萄带毒率较高,病毒复合侵染情况普遍;显症样品中,GFabV检出率为88.2%,GPGV和葡萄浆果内坏死病毒(Grapevine berry inner necrosis virus,GINV)检出率为64.7%和29.4%,均明显高于无症状样品(13.8%和10.3%)。本研究旨在探明‘阳光玫瑰’葡萄携带病毒的种类和侵染状况,为其病毒病防控及病毒脱除奠定基础。

Small RNA sequencing and RT-PCR detection of viruses infecting‘Shine Muscat’grapevines

‘Shine-Muscat’ grapevine is a new introduced cultivar in China. To identify the related viruses with the ‘Shine-Muscat’ grapevine virus disease,we adopted small RNA sequencing to detect the viruses in the asymptomatic and symptomatic ‘Shine-Muscat’ grapevine samples. Results showed that the presence of eight viruses in the symptomatic ‘Shine-Muscat’ grapevine including Grapevine fabavirus (GFabV) and Grapevine Pinot gris virus (GPGV), and only three viruses in the asymptomatic grapevine. Forty-six ‘Shine-Muscat’ grapevine samples were tested for 14 viruses by RT-PCR, and the results showed that these samples had high virus detection rate, and mixed-infection of multiple viruses was common. Grapevine fabavirus (GFabV) was only found in the symptomatic samples with a detection rate of 88.2%. There was a higher incidence of GPGV and Grapevine berry inner necrosis virus (GINV) (64.7% and 29.4%) in symptomatic samples than that in asymptomatic samples (13.8% and 10.3%). This study identified the species and infection status of viruses in ‘Shine-Muscat’ grapevines, and provides the theoretical basis for the control of ‘Shine-Muscat’ grapevine virus disease by sanitary treatments for propagation materials.