| 黄精总皂苷超声提取工艺研究 |
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| 引用本文： | 尤新军,郭蕊,王琳,王俊儒. 黄精总皂苷超声提取工艺研究[J]. 西北林学院学报, 2010, 25(3): 163-166 |
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| 作者姓名： | 尤新军 郭蕊 王琳 王俊儒 |
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| 作者单位： | 西北农林科技大学生命科学学院;西北农林科技大学理学院; |
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| 基金项目： | 2005年度教育部新世纪优秀人才支持计划 |
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| 摘 要： | 采用水浴加热法、超声法和微波法等3种方法提取黄精总皂苷。结果表明:超声法提取黄精总皂苷方法最佳,具有高效、安全、成本低等优点,且能保护被提取成分不被破坏。采用超声提取,以人参皂苷Rb1标准品为指标,通过正交实验确定最佳提取温度、料液比和提取时间。结果表明:提取温度对黄精总皂苷提取有极显著的影响,提取时间和料液比影响不显著。确定最优提取工艺为:超声提取,80%乙醇提取2次,提取温度60℃,提取时间50 min,料液比1∶15,提取总皂苷含量在3.4%以上。
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| 关 键 词： | 黄精 皂苷 提取工艺 正交设计 |
Ultrasonic Extraction Process of Saponins from Polygonatum sibiricun |
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| YOU Xin-jun,GUO Rui,WANG Lin,WANG Jun-ru. Ultrasonic Extraction Process of Saponins from Polygonatum sibiricun[J]. Journal of Northwest Forestry University, 2010, 25(3): 163-166 |
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| Authors: | YOU Xin-jun GUO Rui WANG Lin WANG Jun-ru |
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| Affiliation: | YOU Xin-jun1,GUO Rui1,WANG Lin2,WANG Jun-ru2(1.College of Life Sciences,Northwest A&F University,Yangling,Shaanxi 712100,China,2.College of Sciences,China) |
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| Abstract: | A comparative investigation was carried out on the extraction of saponins from Polygonatum sibiricun with three methods: hot water bath refluxing,ultrasonic wave aided extraction,and microwave aided extraction.Ultrasonic wave aided extraction was selected as an optimal method with the advantages of efficiency,safty,low cost,and non-damage of saponins.Using the content of ginsenoside Rb1 as an indictor,the optimal ultrasonic wave aided extraction conditions were determined as: solvent: 80 % ethanol,times of ... |
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| Keywords: | Polygonatum sibiricum saponins extraction methods orthogonal design |
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