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NaN_3处理条件下西瓜直接再生试验体系研究
引用本文:杨国志,张明方,顾掌根,缑艳霞.NaN_3处理条件下西瓜直接再生试验体系研究[J].长江蔬菜,2009(6).
作者姓名:杨国志  张明方  顾掌根  缑艳霞
作者单位:1. 浙江嘉兴市农科院,314016
2. 浙江大学园艺系
3. 呼和浩特职业技术学院
摘    要:以西瓜纯种自交系伊选为材料,研究了化学诱变剂NaN3对西瓜直接再生试验体系的影响.研究发现,随着叠氯化钠浓度的升高,显著的延迟了种子萌发、胚根发生、子叶开展、不定芽分化、伸长以及生根的时间,降低了芽分化频率和每个外植体分化的不定芽数.1.5 mmol/L的NaN3振荡处理1.5 h为最佳的诱变处理,使外植体再生频率降低到40.0%,不定芽数/外植体降低到4.3,符合抗冷突变体半致死剂量的要求.

关 键 词:化学诱变  不定芽分化  半致死剂量

Study on the Direct Regeneration System of Watermelon under the Treatment of NaN_3
YANG Guozhi,ZHANG Mingfang,GU Zhanggen,GOU Yanxia.Study on the Direct Regeneration System of Watermelon under the Treatment of NaN_3[J].Journal of Changjiang Vegetables,2009(6).
Authors:YANG Guozhi  ZHANG Mingfang  GU Zhanggen  GOU Yanxia
Institution:1.Jiaxing Academy of Agricultural Sciences;Zhejiang 314016;2.Department of Horticulture;Zhejiang University;3.Huhehaote Vocational Technical College
Abstract:Pure watermelon inbred line "Yixuan"was used in this experiment, the effects of NaN3 on the direct regeneration system of watermelon was studied. The results showed that with the increase of NaN3 concentration, the time of germination, radicle elongation, cotyledon extending, adventitious bud differentiation and elongation, and root regeneration was deferred distinctly; the percentage of adventitious bud differentiation and the ratio of adventitious bud differentiation per explant was lowered too. The best ...
Keywords:NaN3
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