| 基于松科树种EST序列的落叶松SSR引物开发 |
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| 引用本文： | 贯春雨,张含国,张磊,朱航勇,邓继峰.基于松科树种EST序列的落叶松SSR引物开发[J].东北林业大学学报,2011,39(1):20-23. |
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| 作者姓名： | 贯春雨 张含国 张磊 朱航勇 邓继峰 |
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| 作者单位： | 东北林业大学,哈尔滨,150040 |
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| 基金项目： | 国家自然科学基金，国家科技基础性工作专项 |
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| 摘 要： | 登陆NCBI的dbEST数据库搜索松科树种EST序列,应用SSRIT软件在线搜索EST-SSR,用Primer3设计EST-SSR引物.选用日本落叶松(L.leptolepis)×兴安落叶松(L.gmelinii)及其145个F1为检测植株,对所开发EST-SSR引物进行扩增,并以丙烯酰胺凝胶检测扩增产物,以获得适合落...
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| 关 键 词： | 松科 落叶松 EST-SSR 引物开发 |
Development of Larix leptolepis SSR Primers from EST Sequence of Pinaceae Family |
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| Guan Chunyu,Zhang Hanguo,Zhang Lei,Zhu Hangyong,Deng Jifeng.Development of Larix leptolepis SSR Primers from EST Sequence of Pinaceae Family[J].Journal of Northeast Forestry University,2011,39(1):20-23. |
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| Authors: | Guan Chunyu Zhang Hanguo Zhang Lei Zhu Hangyong Deng Jifeng |
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| Institution: | Guan Chunyu,Zhang Hanguo,Zhang Lei,Zhu Hangyong,Deng Jifeng(School of Forestry,Northeast Forestry University,Harbin 150040,P.R.China) |
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| Abstract: | A search for EST sequence of Pinaceae species were made by dbEST database of NCBI.SSRIT software was used to search EST-SSR online and primer3 to design EST-SSR primers.Larix leptolepis,L.gmelinii and their 145 full-sib F1 individuals were amplified by the EST-SSR primers,and then the amplified products were detected by acrylamide gel.Suitable SSR primers were selected for larch to construct genetic map and QTL mapping for the family.Through the sequence analysis of 40608 ESTs in Pinaceae deposited in NCBI,... |
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| Keywords: | EST-SSR |
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